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Open AccessArticle

Gentiana lutea Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes

1
Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Breisgau, Germany
2
Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, CZ-50005 Hradec Králové, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Anna Andolfi and Maria Michela Salvatore
Molecules 2020, 25(8), 1832; https://doi.org/10.3390/molecules25081832
Received: 10 March 2020 / Revised: 9 April 2020 / Accepted: 14 April 2020 / Published: 16 April 2020
(This article belongs to the Special Issue Natural Secondary Metabolites)
Gentiana lutea is a bitter herb that is traditionally used to improve gastric disorders. Recently, we have shown that Gentiana lutea extract (GE) also modulates the lipid metabolism of human keratinocytes in vitro and in vivo. In the present study, we investigated the role of GE on ceramide synthesis in human primary keratinocytes (HPKs) and psoriasis-like keratinocytes. We could demonstrate that GE increased the concentrations of glucosylceramides and the ceramide AS/AdS subclass without affecting the overall ceramide content in HPKs. The expression of ceramide synthase 3 (CERS3) and elongases (ELOVL1 and 4) was reduced in psoriasis lesions compared to healthy skin. Psoriasis-like HPKs, generated by stimulating HPKs with cytokines that are involved in the pathogenesis of psoriasis (IL-17, TNF-α, IL-22 and IFN-γ) showed increased levels of IL-6, IL-8 and increased expression of DEFB4A, as well as decreased expression of ELOVL4. The treatment with GE partly rescued the reduced expression of ELOVL4 in psoriasis-like HPKs and augmented CERS3 expression. This study has shown that GE modulates ceramide synthesis in keratinocytes. Therefore, GE might be a novel topical treatment for skin diseases with an altered lipid composition such as psoriasis. View Full-Text
Keywords: ceramides; skin; Gentiana lutea; CerS; ELOVL ceramides; skin; Gentiana lutea; CerS; ELOVL
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MDPI and ACS Style

Gendrisch, F.; Nováčková, A.; Sochorová, M.; Haarhaus, B.; Vávrová, K.; Schempp, C.M.; Wölfle, U. Gentiana lutea Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes. Molecules 2020, 25, 1832.

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