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Anti-Inflammatory Effects of Alnus Sibirica Extract on In Vitro and In Vivo Models

1
Department of Physiology, College of Medicine, Chung-Ang University, Seoul 06974, Korea
2
Laboratory of Pharmacognosy and Natural Product based Medicine, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea
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Red Cross College of Nursing, Chung-Ang University, Seoul 06974, Korea
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Department of Family Medicine, College of Medicine, Chung-Ang University Hospital, 102 Heukseok-ro, Seoul 06973, Korea
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(6), 1418; https://doi.org/10.3390/molecules25061418
Received: 17 February 2020 / Revised: 17 March 2020 / Accepted: 19 March 2020 / Published: 20 March 2020
Alnus sibirica extracts (ASex) have long been used in Oriental medicine to treat various conditions. To provide a scientific basis for this application and the underlying mechanism, we investigated the anti-inflammatory effects of ASex in vitro and in vivo. The in vitro model was established using human dermal fibroblasts (HDFs) treated with inflammatory stimulants (lipopolysaccharide, tumor necrosis factor-alpha, interferon-gamma). Lactate dehydrogenase and reverse transcription-polymerase chain reaction showed that ASex inhibited the increased expression of acute-phase inflammatory cytokines. The in vivo model was established by inducing skin inflammation in NC/Nga mice via the repeated application of house dust mite (HDM) ointment to the ears and back of the mice for eight weeks. HDM application increased the severity of skin lesions, eosinophil/mast cell infiltration, and serum immunoglobulin E levels, which were all significantly decreased by ASex treatment, demonstrating the same degree of protection as hydrocortisone. Overall, ASex showed excellent anti-inflammatory effects both in vitro and in vivo, suggesting its potential as an excellent candidate drug to reduce skin inflammation. View Full-Text
Keywords: Alnus sibirica; skin inflammation; house dust mite ointment (HDM); human dermal fibroblasts (HDFs); inflammatory stimulants; NC/Nga mice Alnus sibirica; skin inflammation; house dust mite ointment (HDM); human dermal fibroblasts (HDFs); inflammatory stimulants; NC/Nga mice
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Choi, J.; Moon, S.; Bae, H.; Kim, Y.-W.; Seo, Y.; Wang, H.S.; Lee, M.W.; Yoo, H.Y.; Kim, J.-H.; Ko, J.-H.; Lim, I.; Bang, H. Anti-Inflammatory Effects of Alnus Sibirica Extract on In Vitro and In Vivo Models. Molecules 2020, 25, 1418.

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