CB1 Receptor-Dependent and Independent Induction of Lipolysis in Primary Rat Adipocytes by the Inverse Agonist Rimonabant (SR141716A)
Abstract
:1. Introduction
2. Results
2.1. Rimonabant Stimulates Lipolysis in Primary Rat Adipocytes
2.2. Rimonabant Stimulates Lipolysis by CB1R-Dependent and Independent Mechanisms
2.3. Rimonabant Stimulates PKA (Protein Kinase A) in Adipocytes
2.4. Rimonabant Increases Accessibility of LD for Lipolytic Cleavage in a Cell-Free System
2.5. Rimonabant Improves Accessibility of LD for Lipolytic Cleavage in Intact Adipocytes
3. Discussion
3.1. The Direct CB1R-Dependent Lipolytic Activity of Rimonabant
3.2. The Direct CB1R-Independent Lipolytic Activity of Rimonabant
3.3. Physiological Relevance of the Direct Lipolytic Activity of Rimonabant
4. Materials and Methods
4.1. Animal Handling and Rimonabant Treatment
4.2. Preparation and Incubation of Primary Rat Adipocytes
4.3. Protein and Lipid Composition of LD from Adipocytes
4.4. Cell-Free Lipolysis System
4.5. Phosphorylation of LD and HSL In Vitro
4.6. Lipid Analysis
4.7. PKA Activity Ratio
4.8. Statistics
4.9. Miscellaneous
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds Rimonabant, AM251, AM281, AM630 and NBD-FA are available from the authors. |
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Müller, G.A.; Herling, A.W.; Wied, S.; Müller, T.D. CB1 Receptor-Dependent and Independent Induction of Lipolysis in Primary Rat Adipocytes by the Inverse Agonist Rimonabant (SR141716A). Molecules 2020, 25, 896. https://doi.org/10.3390/molecules25040896
Müller GA, Herling AW, Wied S, Müller TD. CB1 Receptor-Dependent and Independent Induction of Lipolysis in Primary Rat Adipocytes by the Inverse Agonist Rimonabant (SR141716A). Molecules. 2020; 25(4):896. https://doi.org/10.3390/molecules25040896
Chicago/Turabian StyleMüller, Günter A., Andreas W. Herling, Susanne Wied, and Timo D. Müller. 2020. "CB1 Receptor-Dependent and Independent Induction of Lipolysis in Primary Rat Adipocytes by the Inverse Agonist Rimonabant (SR141716A)" Molecules 25, no. 4: 896. https://doi.org/10.3390/molecules25040896