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Article

Towards the Properties of Different Biomass-Derived Proteins via Various Extraction Methods

Department of Conversion Technologies of Biobased Resources, Institute of Agricultural Engineering, University of Hohenheim, Garbenstrasse 9, 70599 Stuttgart, Germany
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Academic Editor: Rafael Luque Luís Adriano Santos Do Nascimento
Molecules 2020, 25(3), 488; https://doi.org/10.3390/molecules25030488
Received: 30 December 2019 / Revised: 20 January 2020 / Accepted: 21 January 2020 / Published: 23 January 2020
(This article belongs to the Special Issue Advances in Conversion of Biomass and Waste to Chemicals and Fuels)
This study selected three representative protein-rich biomass—brewer’s spent grain (BSG), pasture grass (PG), and cyanobacteria (Arthrospira platensis; AP) for protein extraction with different extraction methods (alkaline treatment, aqueous extraction, and subcritical water extraction). The yield, purity, molecular weight, oil–water interfacial tension, and thermal stability of the obtained proteins derived from different biomass and extraction methods were comprehensively characterized and compared. In the view of protein yield and purity, alkaline treatment was found optimal for BSG (21.4 and 60.2 wt.%, respectively) and AP (55.5 and 68.8 wt.%, respectively). With the decreased oil–water interfacial tension, the proteins from all biomass showed the potential to be emulsifier. BSG and AP protein obtained with chemical treatment presented excellent thermal stability. As a novel method, subcritical water extraction is promising in recovering protein from all three biomass with the comparable yield and purity as alkaline treatment. Furthermore, the hydrolyzed protein with lower molecular weight by subcritical water could promote its functions of foaming and emulsifying. View Full-Text
Keywords: lignocellulosic biomass; protein extraction; subcritical water extraction; protein characterization lignocellulosic biomass; protein extraction; subcritical water extraction; protein characterization
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MDPI and ACS Style

Du, L.; Arauzo, P.J.; Meza Zavala, M.F.; Cao, Z.; Olszewski, M.P.; Kruse, A. Towards the Properties of Different Biomass-Derived Proteins via Various Extraction Methods. Molecules 2020, 25, 488. https://doi.org/10.3390/molecules25030488

AMA Style

Du L, Arauzo PJ, Meza Zavala MF, Cao Z, Olszewski MP, Kruse A. Towards the Properties of Different Biomass-Derived Proteins via Various Extraction Methods. Molecules. 2020; 25(3):488. https://doi.org/10.3390/molecules25030488

Chicago/Turabian Style

Du, Lin, Pablo J. Arauzo, Maria F. Meza Zavala, Zebin Cao, Maciej P. Olszewski, and Andrea Kruse. 2020. "Towards the Properties of Different Biomass-Derived Proteins via Various Extraction Methods" Molecules 25, no. 3: 488. https://doi.org/10.3390/molecules25030488

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