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Protaetia brevitarsis seulensis Derived Protein Isolate with Enhanced Osteomodulatory and Antioxidative Property

1
Department of Biosystems Engineering, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea
2
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2020, 25(24), 6056; https://doi.org/10.3390/molecules25246056
Received: 31 October 2020 / Revised: 13 December 2020 / Accepted: 21 December 2020 / Published: 21 December 2020
(This article belongs to the Special Issue Biomaterials for Hard Tissue Regeneration)
The osteogenic differentiation of stem cells is profoundly affected by their microenvironmental conditions. The differentiation behavior of stem cells can be tuned by changing the niche environments. The proteins or peptides that are derived by living organisms facilitate the osteogenic differentiation of stem cells. Here, we have evaluated the osteoinductive and antioxidative potential of the Protaetia brevitarsis seulensis insect-derived protein for human bone marrow-derived mesenchymal stem cells (hBMSCs). The amino acid contents in the isolated protein were determined by an amino acid analyzer. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to analyze the extract’s functional groups and surface morphology. The extracted protein exhibited 51.08% β-sheet conformation. No adverse effects were observed in extract-treated cells, indicating their biocompatibility. The protein isolate showed an excellent antioxidative property. Besides this, an enhancement in the hBMSCs’ mineralization has been observed in the presence of treated protein isolates. Notably, osteogenic marker genes and proteins were effectively expressed in the treated cells. These results indicated that the P. brevitarsis-derived protein isolate can be used as a potential antioxidative biomaterial for bone tissue engineering applications. View Full-Text
Keywords: Protaetia brevitarsis seulensis; Protaetia protein isolate; biocompatibility; antioxidant; osteogenesis Protaetia brevitarsis seulensis; Protaetia protein isolate; biocompatibility; antioxidant; osteogenesis
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MDPI and ACS Style

Ganguly, K.; Jeong, M.-S.; Dutta, S.D.; Patel, D.K.; Cho, S.-J.; Lim, K.-T. Protaetia brevitarsis seulensis Derived Protein Isolate with Enhanced Osteomodulatory and Antioxidative Property. Molecules 2020, 25, 6056. https://doi.org/10.3390/molecules25246056

AMA Style

Ganguly K, Jeong M-S, Dutta SD, Patel DK, Cho S-J, Lim K-T. Protaetia brevitarsis seulensis Derived Protein Isolate with Enhanced Osteomodulatory and Antioxidative Property. Molecules. 2020; 25(24):6056. https://doi.org/10.3390/molecules25246056

Chicago/Turabian Style

Ganguly, Keya, Min-Soo Jeong, Sayan D. Dutta, Dinesh K. Patel, Seong-Jun Cho, and Ki-Taek Lim. 2020. "Protaetia brevitarsis seulensis Derived Protein Isolate with Enhanced Osteomodulatory and Antioxidative Property" Molecules 25, no. 24: 6056. https://doi.org/10.3390/molecules25246056

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