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Open AccessArticle

Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection

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Postnova Analytics GmbH, Max-Planck-Straße 14, 86899 Landsberg, Germany
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Malvern Panalytical Ltd., Grovewood Road, Malvern, Worcestershire WR14 1XZ, UK
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Authors to whom correspondence should be addressed.
Academic Editor: Sophie Briffa
Molecules 2020, 25(20), 4703; https://doi.org/10.3390/molecules25204703
Received: 21 September 2020 / Revised: 8 October 2020 / Accepted: 12 October 2020 / Published: 14 October 2020
(This article belongs to the Special Issue Nanosafety: Overcoming Characterisation Challenges in Complex Media)
Accurate physico-chemical characterization of exosomes and liposomes in biological media is challenging due to the inherent complexity of the sample matrix. An appropriate purification step can significantly reduce matrix interferences, and thus facilitate analysis of such demanding samples. Electrical Asymmetrical Flow Field-Flow Fractionation (EAF4) provides online sample purification while simultaneously enabling access to size and Zeta potential of sample constituents in the size range of approx. 1–1000 nm. Hyphenation of EAF4 with Multi-Angle Light Scattering (MALS) and Nanoparticle Tracking Analysis (NTA) detection adds high resolution size and number concentration information turning this setup into a powerful analytical platform for the comprehensive physico-chemical characterization of such challenging samples. We here present EAF4-MALS hyphenated with NTA for the analysis of liposomes and exosomes in complex, biological media. Coupling of the two systems was realized using a flow splitter to deliver the sample at an appropriate flow speed for the NTA measurement. After a proof-of-concept study using polystyrene nanoparticles, the combined setup was successfully applied to analyze liposomes and exosomes spiked into cell culture medium and rabbit serum, respectively. Obtained results highlight the benefits of the EAF4-MALS-NTA platform to study the behavior of these promising drug delivery vesicles under in vivo like conditions. View Full-Text
Keywords: liposomes; exosomes; rabbit serum; cell culture medium; protein corona; electrical asymmetrical flow field-flow fractionation; nanoparticle tracking analysis; size separation; absolute number concentration; zeta potential liposomes; exosomes; rabbit serum; cell culture medium; protein corona; electrical asymmetrical flow field-flow fractionation; nanoparticle tracking analysis; size separation; absolute number concentration; zeta potential
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MDPI and ACS Style

Drexel, R.; Siupa, A.; Carnell-Morris, P.; Carboni, M.; Sullivan, J.; Meier, F. Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection. Molecules 2020, 25, 4703. https://doi.org/10.3390/molecules25204703

AMA Style

Drexel R, Siupa A, Carnell-Morris P, Carboni M, Sullivan J, Meier F. Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection. Molecules. 2020; 25(20):4703. https://doi.org/10.3390/molecules25204703

Chicago/Turabian Style

Drexel, Roland; Siupa, Agnieszka; Carnell-Morris, Pauline; Carboni, Michele; Sullivan, Jo; Meier, Florian. 2020. "Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection" Molecules 25, no. 20: 4703. https://doi.org/10.3390/molecules25204703

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