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Article

Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis

Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Catholic University of Daegu, Daegu 42472, Korea
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Author to whom correspondence should be addressed.
Molecules 2020, 25(19), 4517; https://doi.org/10.3390/molecules25194517
Received: 18 August 2020 / Revised: 25 September 2020 / Accepted: 30 September 2020 / Published: 2 October 2020
(This article belongs to the Special Issue Phytochemicals in Medicine and Food)
The present study aims to investigate the immunomodulatory effects of essential oil from Chamaecyparis obtusa (EOCO) in an ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model. BALB/c mice were intraperitoneally sensitized and stimulated with OVA. From day 22 to 35, 0.01% and 0.1% ECOC was intranasally administered 1 h before OVA stimulation. Nasal symptoms, as well as serum total and OVA-specific immunoglobulin (Ig) E levels, were measured. Interleukin (IL)-4, IL-10, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α levels in nasal lavage fluid (NLF) and their production by activated splenocytes were measured. Histological changes in the sinonasal mucosa were evaluated through hematoxylin and eosin and periodic acid-Schiff staining procedure. Th cytokines and their transcription factor mRNA expressions were determined using reverse-transcription polymerase chain reaction. Intranasal EOCO administration significantly suppressed allergic symptoms, OVA-specific IgE level, sinonasal mucosal inflammatory cell infiltration, and mucus-producing periodic acid-Schiff (PAS) positive cell count. EOCO also significantly inhibited IL-4, IL-10, and TNF-α levels in NLF and activated splenocytes. Th2 and Treg related cytokines and their transcription factors in sinonasal mucosa were significantly suppressed through intransal EOCO instillation. In conclusion, repetitive EOCO intranasal instillation showed anti-inflammatory and anti-allergic effects by suppressing nasal symptoms and inhibiting the production and expression of inflammatory mediators in the OVA-induced AR mouse model. View Full-Text
Keywords: allergic rhinitis; essential oil; Chamaecyparis obtusa; cytokine; transcription factor allergic rhinitis; essential oil; Chamaecyparis obtusa; cytokine; transcription factor
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MDPI and ACS Style

Shin, S.-H.; Ye, M.-K.; Lee, D.-W.; Che, M.-H. Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis. Molecules 2020, 25, 4517. https://doi.org/10.3390/molecules25194517

AMA Style

Shin S-H, Ye M-K, Lee D-W, Che M-H. Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis. Molecules. 2020; 25(19):4517. https://doi.org/10.3390/molecules25194517

Chicago/Turabian Style

Shin, Seung-Heon, Mi-Kyung Ye, Dong-Won Lee, and Mi-Hyun Che. 2020. "Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis" Molecules 25, no. 19: 4517. https://doi.org/10.3390/molecules25194517

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