Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica
Abstract
:1. Introduction
2. Results and Discussion
2.1. Growth and Biosurfactant Production
2.1.1. Haemolytic Activity
2.1.2. Drop Collapse Test
2.1.3. Oil-Spreading Test
2.1.4. Surface Tension Measurement
2.1.5. Emulsification Activity
2.1.6. Microbial Adhesion to Hydrocarbons (MATH) Assay
2.1.7. Stability of Biosurfactant
2.2. Optimisation of Crude Biosurfactant Production
2.3. Growth on Canola Oil Modelled Using Secondary Models
3. Materials and Methods
3.1. Microorganism and Shake Flask Culture Condition
3.2. Biosurfactant Analysis
3.2.1. Haemolytic Activity
3.2.2. Drop Collapse Test
3.2.3. Oil-Spreading Test
3.2.4. Emulsification Index Test
3.2.5. Surface Tension
3.2.6. Microbial Adhesion to Hydrocarbon (MATH) Assay
3.2.7. Biosurfactant Stability Test
3.2.8. Optimisation of Biosurfactant Production
3.2.9. Biosurfactant Extraction
3.3. Bacterial Growth Kinetics Modelling
Model | Equation | No. of Parameters | References |
---|---|---|---|
Monod | 2 | [54] | |
Haldane | 3 | [55] | |
Teissier | 3 | [56] | |
Luong | 4 | [57] | |
Aiba | 4 | [58] | |
Yano and Koga | 4 | [59] | |
Webb | 4 | [60] |
Verification of the Models
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Model | Parameter | R2 | ADJ R2 | AICc | RMSE | SSE | BF | AF |
---|---|---|---|---|---|---|---|---|
Haldane | 3 | 0.9547 | 0.9364 | −105.46 | 0.004733 | 0.000162 | 1.00 | 1.07 |
Teissier | 3 | 0.8971 | 0.8677 | −92.29 | 0.006949 | 0.000338 | 1.00 | 1.04 |
Monod | 2 | 0.7181 | 0.8272 | −90.38 | 0.01138 | 0.001166 | 0.98 | 1.12 |
Yano | 4 | 0.9464 | 0.9196 | −102.10 | 0.005418 | 0.000176 | 1.00 | 1.07 |
Luong | 4 | 0.9064 | 0.8797 | −94.68 | 0.006626 | 0.000301 | 1.00 | 1.08 |
Aiba | 3 | 0.9333 | 0.9661 | −101.57 | 0.005592 | 0.000219 | 1.00 | 1.09 |
Webb | 4 | 0.9646 | 0.8520 | −103.61 | 0.007349 | 0.000324 | 1.01 | 1.08 |
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Ibrahim, S.; Abdul Khalil, K.; Zahri, K.N.M.; Gomez-Fuentes, C.; Convey, P.; Zulkharnain, A.; Sabri, S.; Alias, S.A.; González-Rocha, G.; Ahmad, S.A. Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules 2020, 25, 3878. https://doi.org/10.3390/molecules25173878
Ibrahim S, Abdul Khalil K, Zahri KNM, Gomez-Fuentes C, Convey P, Zulkharnain A, Sabri S, Alias SA, González-Rocha G, Ahmad SA. Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules. 2020; 25(17):3878. https://doi.org/10.3390/molecules25173878
Chicago/Turabian StyleIbrahim, Salihu, Khalilah Abdul Khalil, Khadijah Nabilah Mohd Zahri, Claudio Gomez-Fuentes, Peter Convey, Azham Zulkharnain, Suriana Sabri, Siti Aisyah Alias, Gerardo González-Rocha, and Siti Aqlima Ahmad. 2020. "Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica" Molecules 25, no. 17: 3878. https://doi.org/10.3390/molecules25173878