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Open AccessArticle

Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China

Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
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Academic Editors: Pierluigi Luigi Mauri, Martina Marchetti-Deschmann and Diana Canetti
Molecules 2020, 25(16), 3605; https://doi.org/10.3390/molecules25163605
Received: 23 July 2020 / Revised: 5 August 2020 / Accepted: 7 August 2020 / Published: 8 August 2020
(This article belongs to the Special Issue Mass Spectrometric Proteomics II)
Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality. View Full-Text
Keywords: Dipsacus asperoides; iTRAQ; proteomic Dipsacus asperoides; iTRAQ; proteomic
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MDPI and ACS Style

Jin, H.; Yu, H.; Wang, H.; Zhang, J. Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China. Molecules 2020, 25, 3605.

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