Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods
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Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Akademicheskaya St. 27, Minsk 220072, Belarus
2
Institute of Atomic Physics and Spectroscopy, University of Latvia, 19, Raina Blvd, 1586 Riga, Latvia
3
Center for Collective Use of Scientific Equipment, Sumy State University, 31, Sanatornaya str., 40018 Sumy, Ukraine
4
NanoBioMedical Centre, Adam Mickiewicz University in Poznan, Wszechnicy Piastowskiej str. 3, 61-614 Poznan, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Stefan Jurga and Dorota Flak
Molecules 2020, 25(14), 3168; https://doi.org/10.3390/molecules25143168
Received: 3 June 2020
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Revised: 3 July 2020
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Accepted: 8 July 2020
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Published: 10 July 2020
(This article belongs to the Special Issue NanoTech Poland 2021—Nanotechnology in Energy, Electronics, Photonics, Environment and Biomedicine)
The precise detection of cancer cells currently remains a global challenge. One-dimensional (1D) semiconductor nanostructures (e.g., ZnO nanorods) have attracted attention due to their potential use in cancer biosensors. In the current study, it was demonstrated that the possibility of a photoluminescent detection of human leukemic T-cells by using a zinc oxide nanorods (ZnO NRs) platform. Monoclonal antibodies (MABs) anti-CD5 against a cluster of differentiation (CD) proteins on the pathologic cell surface have been used as a bioselective layer on the ZnO surface. The optimal concentration of the protein anti-CD5 to form an effective bioselective layer on the ZnO NRs surface was selected. The novel biosensing platforms based on glass/ZnO NRs/anti-CD5 were tested towards the human T-lymphoblast cell line MOLT-4 derived from patients with acute lymphoblastic leukemia. The control tests towards MOLT-4 cells were performed by using the glass/ZnO NRs/anti-IgG2a system as a negative control. It was shown that the photoluminescence signal of the glass/ZnO NRs/anti-CD5 system increased after adsorption of T-lymphoblast MOLT-4 cells on the biosensor surface. The increase in the ZnO NRs photoluminescence intensity correlated with the number of CD5-positive MOLT-4 cells in the investigated population (controlled by using flow cytometry). Perspectives of the developed ZnO platforms as an efficient cancer cell biosensor were discussed.
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Keywords:
zinc oxide nanorods; MOLT-4 cell line; T-lymphoblasts detection; cluster of differentiation proteins; monoclonal antibody anti-CD5; room temperature photoluminescence
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MDPI and ACS Style
Tamashevski, A.; Harmaza, Y.; Slobozhanina, E.; Viter, R.; Iatsunskyi, I. Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods. Molecules 2020, 25, 3168. https://doi.org/10.3390/molecules25143168
AMA Style
Tamashevski A, Harmaza Y, Slobozhanina E, Viter R, Iatsunskyi I. Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods. Molecules. 2020; 25(14):3168. https://doi.org/10.3390/molecules25143168
Chicago/Turabian StyleTamashevski, Alexander, Yuliya Harmaza, Ekaterina Slobozhanina, Roman Viter, and Igor Iatsunskyi. 2020. "Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods" Molecules 25, no. 14: 3168. https://doi.org/10.3390/molecules25143168
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