Next Article in Journal
Enzymes, Biocatalysis and Chemical Biology
Previous Article in Journal
Mechanisms Regulating the UPS-ALS Crosstalk: The Role of Proteaphagy
Open AccessArticle

Sexual Dimorphism in Energy Metabolism of Wistar Rats Using Data Analysis

1
Institute of Biology and Ecology, Faculty of Sciences, University of Pavol Jozef Šafárik in Košice, Šrobárova 2, 041 80 Košice, Slovakia
2
Department of Informatics, Faculty of Management Sciences and Informatics, University of Žilina, Univerzitná 8215/1, 010 26 Žilina, Slovakia
3
Department of Pathology, Faculty of Medicine, University of Pavol Jozef Šafárik in Košice, Rastislavova 43, 040 01 Košice, Slovakia
*
Authors to whom correspondence should be addressed.
Molecules 2020, 25(10), 2353; https://doi.org/10.3390/molecules25102353
Received: 9 April 2020 / Revised: 6 May 2020 / Accepted: 11 May 2020 / Published: 18 May 2020
(This article belongs to the Section Analytical Chemistry)
The prevalence of some chronic diseases, such as cancer or neurodegenerative disorders, differs between sexes. Animal models provide an important tool to adopt potential therapies from preclinical studies to humans. Laboratory rats are the most popular animals in toxicology, neurobehavioral, or cancer research. Our study aimed to reveal the basic differences in blood metabolome (amino acids, biogenic amines, and acylcarnitines) of the adult male (n = 10) and female (n = 10) Wistar rats. Partial least square-discrimination analysis (PLS-DA) and a variance im portance in projection (VIP) score was used to identify the key sex-specific metabolites. All groups of metabolites, as the main markers of energy metabolism, showed a significant sex-dependent pattern. The most important features calculated in PLS-DA according to VIP score were free carnitine (C0), tyrosine (Tyr), and acylcarnitine C5-OH. While aromatic amino acids, such as Tyr and phenylalanine (Phe), were significantly elevated in the blood plasma of males, tryptophan (Trp) was found in higher levels in the blood plasma of females. Besides, significant sex-related changes in urea cycle were found. Our study provides an important insight into sex-specific differences in energy metabolism in rats and indicates that further studies should consider sex as the main aspect in design and data interpretation. View Full-Text
Keywords: metabolomics; FIA-MS/MS and LC-MS/MS; energy metabolism; sexual dimorphism; acylcarnitines; amino acids metabolomics; FIA-MS/MS and LC-MS/MS; energy metabolism; sexual dimorphism; acylcarnitines; amino acids
Show Figures

Figure 1

MDPI and ACS Style

Leskanicova, A.; Chovancova, O.; Babincak, M.; Verboova, L.; Benetinova, Z.; Macekova, D.; Kostolny, J.; Smajda, B.; Kiskova, T. Sexual Dimorphism in Energy Metabolism of Wistar Rats Using Data Analysis. Molecules 2020, 25, 2353.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop