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Open AccessArticle

Modulating Heterologous Pathways and Optimizing Culture Conditions for Biosynthesis of trans-10, cis-12 Conjugated Linoleic Acid in Yarrowia lipolytica

by Xun Wang 1,2,3, Qianjun Xia 1,2,3, Fei Wang 1,2,3, Yu Zhang 1,2,3 and Xun Li 1,2,3,*
1
Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-Forest Biomass, Nanjing Forestry University, Nanjing 210037, China
2
Jiangsu Key Laboratory of Biomass-Based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China
3
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(9), 1753; https://doi.org/10.3390/molecules24091753
Received: 7 April 2019 / Revised: 25 April 2019 / Accepted: 30 April 2019 / Published: 6 May 2019
A novel recombinant strain has been constructed for converting glycerol into a specific conjugated linoleic acid isomer (trans-10, cis-12 CLA) using Yarrowia lipolytica as host. The lipid accumulation pathway was modified for increasing lipid content. Overexpression of the diacylglycerol transferase (DGA1) gene improved the intracellular lipid yield by approximately 45% as compared to the original strain. The corresponding intracellular lipid yield of recombinant strain WXYL037 reached 52.2% of the cell dry weight. In combination with integration of Δ12 desaturase from Mortierella alpina (MA12D) and DGA1, the linoleic acid (LA) production content reached 0.88 g/L, which was 2-fold that of the original strain. Furthermore, with overexpressed DGA1, MA12D and Propionibacterium acnes isomerase (PAI), the titer of trans-10, cis-12 CLA in WXYL037 reached 110.6 mg/L after 72 h of shake flask culture, representing a 201.8% improvement when compared with that attained in the WXYL030 strain, which manifested overexpressed PAI. With optimal medium, the maximum CLA content and lipid yield of Y. lipolytica Po1g were 132.6 mg/L and 2.58 g/L, respectively. This is the first report of the production of trans-10, cis-12 CLA by the oleaginous yeast Y. lipolytica using glycerol as the sole carbon source through expression of DGA1 combined with MA12D and PAI. View Full-Text
Keywords: conjugated linoleic acid; metabolic engineering; Yarrowia lipolytica; Propionibacterium acnes isomerase; Mortierella alpine Δ12 desaturase conjugated linoleic acid; metabolic engineering; Yarrowia lipolytica; Propionibacterium acnes isomerase; Mortierella alpine Δ12 desaturase
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MDPI and ACS Style

Wang, X.; Xia, Q.; Wang, F.; Zhang, Y.; Li, X. Modulating Heterologous Pathways and Optimizing Culture Conditions for Biosynthesis of trans-10, cis-12 Conjugated Linoleic Acid in Yarrowia lipolytica. Molecules 2019, 24, 1753.

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