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In Vitro Effects on Thrombin of Paris Saponins and In Vivo Hemostatic Activity Evaluation of Paris fargesii var. brevipetala

1
West China School of Pharmacy, Sichuan University, Chengdu 610041, China
2
Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China
3
College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(7), 1420; https://doi.org/10.3390/molecules24071420
Received: 17 March 2019 / Revised: 4 April 2019 / Accepted: 7 April 2019 / Published: 11 April 2019
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Abstract

The resource shortage of Rhizoma Paridis has never been effectively addressed, and the industry continues to search for alternative resources. The in vitro effects on thrombin of Paris saponins and in vivo hemostatic activity of Paris fargesii var. brevipetala (PF) were evaluated in this study. PF is considered to be an alternative source of Rhizoma Paridis (RP). The in vitro incubation experiment was designed to investigate the effects on thrombin activity of Paris saponin H (PS H) and saponin extract in PF. The bleeding time of mouse tail snipping was used to evaluate the in vivo hemostatic effects of Paris saponins. Also, in vivo changes in four blood coagulation parameters in rats after oral administration of different groups of Paris saponins were compared. The effects of Paris saponins on liver function and blood lipid parameters were examined in order to avoid drug-induced liver injury. Activity studies of thrombin after ultra-filtration centrifugation showed that Paris saponins were able to enhance thrombin activity. Ultra performance liquid chromatography mass spectrometry (UPLC-MS) analysis results of the substrates led us to speculate that there is a specific binding between Paris saponins and thrombin. PS H and Paris saponins in PF significantly shortened the bleeding time in mice. One pathway by which Paris saponins enhance in vivo blood coagulation is by increasing fibrinogen (FIB), among the four blood coagulation parameters in rats. At the same time, the effects on liver and blood lipid parameters were insignificant. P. fargesii var. brevipetala can be developed as an alternative medicinal source of Rhizoma Paridis. View Full-Text
Keywords: Rhizoma Paridis; Paris saponin H; Paris fargesii var. brevipetala; thrombin; hemostatic activity; UPLC-MS Rhizoma Paridis; Paris saponin H; Paris fargesii var. brevipetala; thrombin; hemostatic activity; UPLC-MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Wen, F.; Chen, T.; Yin, H.; Lin, J.; Zhang, H. In Vitro Effects on Thrombin of Paris Saponins and In Vivo Hemostatic Activity Evaluation of Paris fargesii var. brevipetala. Molecules 2019, 24, 1420.

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