A Non-Enzymatic and Label-Free Fluorescence Bioassay for Ultrasensitive Detection of PSA
Abstract
:1. Introduction
2. Results and Discussion
2.1. Principle and Feasibility of the Assay
2.2. Optimization of Reaction Conditions
2.3. Sensitivity and Specificity for PSA Detection
2.4. Determination of PSA in Real Samples
3. Experimental Section
3.1. Reagents and Materials
3.2. Apparatus and Measurements
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available. |
Method | Linear Range | Detection Limit | Reference |
---|---|---|---|
Microfluidic Devices | 10–100 ng/mL | 10 ng/mL | [5] |
Rresonance light scattering | 0.13–110 ng/mL | 0.13 ng/mL | [4] |
Immunosensor | 0.05–26 ng/mL | 0.05 ng/mL | [30] |
electrogenerated chemiluminescence immunosensor | 8–10 pg/mL | 8 pg/mL | [31] |
quartz crystal microbalance sensor | 0.29–150 ng/mL | 0.29 ng/mL | [32] |
Electrochemistry | 0.05–100 ng/mL | 1000 pg/mL | [33] |
Field effect transistor | 0.023–500 ng/mL | 23 pg/mL | [34] |
Fluoroimmunoassay | 0.1–200 ng/mL | 10 pg/mL | This Work |
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Sun, Y.; Wang, C.; Zhang, H.; Zhang, Y.; Zhang, G. A Non-Enzymatic and Label-Free Fluorescence Bioassay for Ultrasensitive Detection of PSA. Molecules 2019, 24, 831. https://doi.org/10.3390/molecules24050831
Sun Y, Wang C, Zhang H, Zhang Y, Zhang G. A Non-Enzymatic and Label-Free Fluorescence Bioassay for Ultrasensitive Detection of PSA. Molecules. 2019; 24(5):831. https://doi.org/10.3390/molecules24050831
Chicago/Turabian StyleSun, Yujie, Chenyun Wang, Hong Zhang, Yulin Zhang, and Guojun Zhang. 2019. "A Non-Enzymatic and Label-Free Fluorescence Bioassay for Ultrasensitive Detection of PSA" Molecules 24, no. 5: 831. https://doi.org/10.3390/molecules24050831