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Amylase and Xylanase from Edible Fungus Neurospora intermedia: Production and Characterization

Swedish Centre for Resource Recovery, University of Borås, SE-50190 Borås, Sweden
Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman 7618868366, Iran
Department of Chemical and Polymer Engineering, Faculty of Engineering, Yazd University, Yazd 8915818411, Iran
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 71345, Iran
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz 7146864685, Iran
Author to whom correspondence should be addressed.
Academic Editor: Kira J. Weissman
Molecules 2019, 24(4), 721;
Received: 24 January 2019 / Revised: 13 February 2019 / Accepted: 16 February 2019 / Published: 17 February 2019
(This article belongs to the Special Issue Biotechnology of Microbial Enzymes)
PDF [1393 KB, uploaded 17 February 2019]


Integrated enzyme production in the biorefinery can significantly reduce the cost of the entire process. The purpose of the present study is to evaluate the production of two hydrolyzing enzymes (amylase and xylanase) by an edible fungus used in the biorefinery, Neurospora intermedia. The enzyme production was explored through submerged fermentation of synthetic media and a wheat-based waste stream (thin stillage and wheat bran). The influence of a nitrogen source on N. intermedia was investigated and a combination of NaNO3 and yeast extract has been identified as the best nitrogen source for extracellular enzyme production. N. intermedia enzymes showed maximum activity at 65 °C and pH around 5. Under these conditions, the maximum velocity of amylase and xylanase for starch and xylan hydrolysis was found to be 3.25 U mL−1 and 14.77 U mL−1, respectively. Cultivation of N. intermedia in thin stillage and wheat bran medium resulted in relatively high amylase (8.86 ± 0.41 U mL−1, 4.68 ± 0.23) and xylanase (5.48 ± 0.21, 2.58 ± 0.07 U mL−1) production, respectively, which makes this fungus promising for enzyme production through a wheat-based biorefinery. View Full-Text
Keywords: amylase; xylanase; Neurospora intermedia; submerged fermentation; wheat-based biorefinery amylase; xylanase; Neurospora intermedia; submerged fermentation; wheat-based biorefinery

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Shahryari, Z.; Fazaelipoor, M.H.; Ghasemi, Y.; Lennartsson, P.R.; Taherzadeh, M.J. Amylase and Xylanase from Edible Fungus Neurospora intermedia: Production and Characterization. Molecules 2019, 24, 721.

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