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Molecules 2019, 24(3), 604; https://doi.org/10.3390/molecules24030604

Evaluation of MMP Inhibitors Isolated from Ligustrum japonicum Fructus

1
Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 49112, Korea
2
Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Baegyang-daero 700beon-gil 140, Sasang-gu, Busan 46958, Korea
3
Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Baegyang-daero 700beon-gil 140, Sasang-gu, Busan 46958, Korea
*
Author to whom correspondence should be addressed.
Received: 7 January 2019 / Revised: 24 January 2019 / Accepted: 31 January 2019 / Published: 8 February 2019
(This article belongs to the Section Natural Products Chemistry)
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Abstract

The current study investigated the ability of two secoiridoids, GL-3 (1) and oleonuezhenide (2), isolated from the fruits of Ligustrum japonicum to inhibit MMP-2 and -9 activity in phorbol 12-myristate 13-acetate (PMA)-induced HT-1080 human fibrosarcoma cells. Both compounds 1 and 2 were able to exert lowered gelatin digestion activity for MMP-2 and -9 tested by gelatin zymography via suppressing the release of MMPs to culture medium according to ELISA results. Treatment with compounds was also able to suppress the expression of both mRNA and protein levels of MMP-2 and -9. Action mechanism behind the MMP inhibitory effect of the compounds was suggested to be via MAPK pathway indicated by decreased levels of phosphorylated p38, ERK and JNK proteins evaluated employing immunoblotting. Compound 1 was shown to be slightly more active to inhibit MMP-2 and -9, however, compound 2 showed more regular dose-dependency during inhibition. In conclusion, this study suggested that GL-3 and oleonuezhenide were notable natural origin potent MMP inhibitors and could serve as lead compounds for development of anti-invasive MMP inhibitors against tumor metastasis. View Full-Text
Keywords: anti-invasive; HT-1080; Ligustrum japonicum fructus; MAPK; MMP anti-invasive; HT-1080; Ligustrum japonicum fructus; MAPK; MMP
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Kim, H.; Karadeniz, F.; Kong, C.-S.; Seo, Y. Evaluation of MMP Inhibitors Isolated from Ligustrum japonicum Fructus. Molecules 2019, 24, 604.

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