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Open AccessArticle

Fingerprint Analysis of Cnidium monnieri (L.) Cusson by High-Speed Counter-Current Chromatography

Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China
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Co-first authors: These authors contributed equally to this work and should be considered co-first authors.
Molecules 2019, 24(24), 4496; https://doi.org/10.3390/molecules24244496
Received: 25 November 2019 / Revised: 6 December 2019 / Accepted: 6 December 2019 / Published: 8 December 2019
Cnidium monnieri (L.) Cusson is a popular Traditional Chinese Medicine (TCM) with a variety of bioactivities. However, there are some problems that have affected the development of Cnidium monnieri (L.) Cusson. At present, many methods have been reported for the analysis of coumarins in Cnidium monnieri (L.) Cusson. However, the quality control of coumarins in Cnidium monnieri (L.) Cusson by high-speed counter-current chromatography (HSCCC) has not been reported. In this study, analytical high-speed counter-current chromatography (HSCCC) was successfully used for fingerprint of Cnidium monnieri (L.) Cusson with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at 4:6:6.5:3.5 (v/v). The UV wavelength was set at 254 nm. Six coumarin compounds with high biological activity were selected as indicator compounds for the quality control. The HSCCC fingerprint of the Cnidium monnieri (L.) Cusson was successfully established and there were some differences according to the results of the fingerprint analysis. The present results demonstrate that HSCCC is an established and efficient technique for the fingerprint analysis of Cnidium monnieri (L.) Cusson and can be used to control the quality of Cnidium monnieri (L.) Cusson. In brief, HSCCC is a useful technology for the fingerprint analytical method for TCM. View Full-Text
Keywords: fingerprint; high-speed counter-current chromatography (HSCCC); Cnidium monnieri (L.) Cusson; traditional Chinese medicine (TCM); quality control fingerprint; high-speed counter-current chromatography (HSCCC); Cnidium monnieri (L.) Cusson; traditional Chinese medicine (TCM); quality control
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Wu, X.; Gao, X.; Zhu, X.; Zhang, S.; Liu, X.; Yang, H.; Song, H.; Chen, Q. Fingerprint Analysis of Cnidium monnieri (L.) Cusson by High-Speed Counter-Current Chromatography. Molecules 2019, 24, 4496.

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