Surface-Enhanced IR-Absorption Microscopy of Staphylococcus aureus Bacteria on Bactericidal Nanostructured Si Surfaces
Abstract
:1. Introduction
2. Experimental Results
2.1. IR Characterization of Bactericidal Si Nanoripples and Nanoparticle Coatings
2.2. IR Characterization of Staphylococcus Aureus Bacterial Biofilms on Bactericidal Si Nanoripples and Nanoparticle Coatings
- in the window between 3000 and 2800 cm−1 (W1, the ‘fatty acid region I’), dominated by the -CH3, >CH, and =CH stretching vibrations of the functional groups usually present in the fatty acid components of the various membrane amphiphiles;
- in the window between 1800 and 1500 cm−1 (W2, the ‘amide region’), dominated by the amide I and amide II bands of proteins and peptides;
- in the window between 1500 and 1200 cm−1 (W3, the ‘mixed region’), a spectral region containing information from proteins, fatty acids and phosphate-carrying compounds, including the window between 1500 and 1400 cm−1 (W31, the ‘fatty acid region II’), dominated by the -CH3 and -CH2 bending vibrations of the same functional groups as expressed in W1;
- in the window between 1200 and 900 cm−1 (W4, the ‘polysaccharide region’), dominated by the fingerprint-like absorption bands of the carbohydrates present within the cell wall.
3. Concluding Remarks
4. Materials and Methods
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Structure/Chemical Composition (at. %) | Silicon Si | Oxygen O | Carbon C | Sulfur S |
---|---|---|---|---|
Si ripples (air) | 67 ± 1 | 15 ± 1 | 17 ± 1 | 0 |
Si nanoripples (CS2) | 75 ± 1 | 10 ± 1 | 14 ± 1 | 0.9 ± 0.3 |
Si nanoripples (H2O) | 92 ± 1 | 2 ± 1 | 6 ± 1 | 0 |
Si nanoparticles (H2O) | 84 ± 1 | 16 ± 1 | 0 | 0 |
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Kudryashov, S.I.; Nastulyavichus, A.A.; Tolordava, E.R.; Kirichenko, A.N.; Saraeva, I.N.; Rudenko, A.A.; Romanova, Y.M.; Panarin, A.Y.; Ionin, A.A.; Itina, T.E. Surface-Enhanced IR-Absorption Microscopy of Staphylococcus aureus Bacteria on Bactericidal Nanostructured Si Surfaces. Molecules 2019, 24, 4488. https://doi.org/10.3390/molecules24244488
Kudryashov SI, Nastulyavichus AA, Tolordava ER, Kirichenko AN, Saraeva IN, Rudenko AA, Romanova YM, Panarin AY, Ionin AA, Itina TE. Surface-Enhanced IR-Absorption Microscopy of Staphylococcus aureus Bacteria on Bactericidal Nanostructured Si Surfaces. Molecules. 2019; 24(24):4488. https://doi.org/10.3390/molecules24244488
Chicago/Turabian StyleKudryashov, Sergey I., Alena A. Nastulyavichus, Eteri R. Tolordava, Alexey N. Kirichenko, Irina N. Saraeva, Andrey A. Rudenko, Yulia M. Romanova, Andrey Yu. Panarin, Andrey A. Ionin, and Tatiana E. Itina. 2019. "Surface-Enhanced IR-Absorption Microscopy of Staphylococcus aureus Bacteria on Bactericidal Nanostructured Si Surfaces" Molecules 24, no. 24: 4488. https://doi.org/10.3390/molecules24244488