Comparative Assessment of Phenolic Profiles, Cellular Antioxidant and Antiproliferative Activities in Ten Varieties of Sweet Potato (Ipomoea Batatas) Storage Roots
Abstract
:1. Introduction
2. Results
2.1. Total Phenolic Content in Different Varieties
2.2. Phytochemical Composition of Ten Varieties
2.3. Total Antioxidant Activity
2.4. Cellular Antioxidant Activity
2.5. Antiproliferative Activity against Human Liver Cancer HepG2 Cell
2.6. Correlation Analysis among Phytochemicals and Antioxidant and Proliferative Activities
3. Discussion
3.1. Comparison of Total Phenolics Content in Different Varieties
3.2. Comparison of Phytochemical Composition and PCA Analysis of Ten Varieties
3.3. Comparison of Total Antioxidant Activity
3.4. Comparison of Cellular Antioxidant Activity
3.5. Comparison of Antiproliferative Activity against Human Liver Cancer HepG2 Cell
4. Materials and Methods
4.1. Sweet Potato Samples Preparation
4.2. Phenolic Extraction for Sweet Potato
4.3. Determination of Phenolics for Sweet Potato Extracts
4.4. Phenolic Profiles of Sweet Potato Extracts Analysis by HPLC-PAD
4.5. Total Antioxidant Activity Analysis for Sweet Potato Extracts
4.6. Cellular Antioxidant Activity Analysis for Sweet Potato Extracts
4.7. Determination of Antiproliferative and Cytotoxicity Activities of Sweet Potato Extracts
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Identified Compound | YS7 | HX22 | YS43 | WS7 | YS25 | YZ7 | YY153 | CS1 | XY34 | YS15 |
---|---|---|---|---|---|---|---|---|---|---|
chlorogenic acid | ND | 20.11 ± 1.68a | 5.34 ± 0.32b | 0.51 ± 0.01c | 0.67 ± 0.01c | ND | ND | ND | 1.37 ± 0.09c | ND |
caffeic acid | ND | 0.56 ± 0.002cd | 4.57 ± 1.18a | 0.96 ± 0.08bc | 0.59 ± 0.05cd | 1.39 ± 0.001b | 0.93 ± 0.01bc | 0.13 ± 0.01d | 1.55 ± 0.01b | 0.10 ± 0.01d |
syringic acid | ND | ND | ND | 0.73 ± 0.11a | 0.74 ± 0.13a | ND | 0.67 ± 0.02a | ND | ND | ND |
cumaric acid | ND | ND | ND | 1.48 ± 0.08a | 1.46 ± 0.02a | ND | ND | ND | 1.46 ± 0.01a | ND |
ferulic acid | 19.13 ± 0.24d | 8.25 ± 0.05g | ND | 18.62 ± 0.01e | 10.86 ± 0.45f | ND | 26.03 ± 0.28b | 23.98 ± 0.04c | 27.97 ± 0.13a | 1.31 ± 0.003h |
trans-ferulic acid | ND | ND | 1.34 ± 0.03a | ND | ND | 0.58 ± 0.02b | ND | ND | ND | ND |
isoquercetin | ND | ND | ND | ND | 0.43 ± 0.03b | ND | 0.41 ± 0.01c | ND | 0.60 ± 0.01a | ND |
benzoic acid | 12.37 ± 2.60 | ND | ND | ND | ND | ND | ND | ND | ND | ND |
isorhamnetin | ND | ND | ND | ND | 2.13 ± 0.19b | ND | 2.25 ± 0.02a | 0.81 ± 0.03c | 0.87 ± 0.04c | ND |
hyperoside | ND | ND | 417.6 ± 14.1b | 7.94 ± 0.97e | ND | 14.78 ± 1.75cd | 471.3 ± 2.5a | 16.34 ± 1.10c | 11.13 ± 1.24cde | 9.26 ± 0.89de |
catechin | ND | ND | ND | ND | ND | 2.15 ± 0.17a | ND | ND | ND | ND |
epicatechin | ND | ND | ND | 0.17 ± 0.001c | ND | ND | 0.30 ± 0.004b | ND | 0.35 ± 0.02a | ND |
coumarin | ND | 0.26 ± 0.01b | ND | 0.23 ± 0.001b | 0.03 ± 0.01d | 0.04 ± 0.004c | ND | ND | 0.34 ± 0.02a | ND |
rutin hydrate | 10.53 ± 2.51b | ND | 33.38 ± 3.09a | ND | ND | ND | ND | ND | ND | ND |
Total Antioxidant Activities (mmol TE/100 g FW) | Cellular Antioxidant Activities (μmol QE/100 g FW) | Contribution (%) | Cyctoxicity (mg/mL) | |
---|---|---|---|---|
Varieties | ORAC | PBS Wash | PBS Wash/ORAC | CC10 |
YS7 | 0.45 ± 0.07d | 3.34 ± 0.48b | 0.74 | 0.698 |
HX22 | 1.73 ± 0.03cd | 30.15 ± 5.87b | 1.74 | 1.993 |
YS43 | 7.38 ± 0.98a | 120.68 ± 36.9a | 1.64 | 1.662 |
WS7 | 1.17 ± 0.18d | 38.47 ± 8.57b | 3.29 | 1.977 |
YS25 | 1.90 ± 0.28cd | 23.14 ± 3.86b | 1.22 | 2.013 |
YZ7 | 3.39 ± 0.36b | 50.73 ± 7.56b | 1.50 | 2.478 |
YY153 | 2.38 ± 0.18bc | 35.22 ± 6.97b | 1.48 | 5.304 |
CS1 | 0.40 ± 0.03d | 0.46 ± 0.08b | 0.12 | ND |
XY34 | 1.19 ± 0.15d | 181.15 ± 18.24a | 15.22 | ND |
YS15 | 0.52 ± 0.04d | ND | ND | ND |
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Sun, Y.; Pan, Z.; Yang, C.; Jia, Z.; Guo, X. Comparative Assessment of Phenolic Profiles, Cellular Antioxidant and Antiproliferative Activities in Ten Varieties of Sweet Potato (Ipomoea Batatas) Storage Roots. Molecules 2019, 24, 4476. https://doi.org/10.3390/molecules24244476
Sun Y, Pan Z, Yang C, Jia Z, Guo X. Comparative Assessment of Phenolic Profiles, Cellular Antioxidant and Antiproliferative Activities in Ten Varieties of Sweet Potato (Ipomoea Batatas) Storage Roots. Molecules. 2019; 24(24):4476. https://doi.org/10.3390/molecules24244476
Chicago/Turabian StyleSun, Yiming, Zhijun Pan, Chunxian Yang, Zhenzhen Jia, and Xinbo Guo. 2019. "Comparative Assessment of Phenolic Profiles, Cellular Antioxidant and Antiproliferative Activities in Ten Varieties of Sweet Potato (Ipomoea Batatas) Storage Roots" Molecules 24, no. 24: 4476. https://doi.org/10.3390/molecules24244476