Next Article in Journal
Cytotoxicity of Triterpene Seco-Acids from Betula pubescens Buds
Next Article in Special Issue
Chia Seeds (Salvia Hispanica L.): An Overview—Phytochemical Profile, Isolation Methods, and Application
Previous Article in Journal
Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity
Previous Article in Special Issue
Separation, Identification, and Antioxidant Activity of Polyphenols from Lotus Seed Epicarp
Open AccessArticle

Antimicrobial Effect of Picea abies Extracts on E. coli Growth

Faculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 1, 39100 Bolzano, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Vincenzo De Feo and Raphaël E. Duval
Molecules 2019, 24(22), 4053; https://doi.org/10.3390/molecules24224053
Received: 1 October 2019 / Revised: 4 November 2019 / Accepted: 6 November 2019 / Published: 9 November 2019
This study aims to investigate the effect of essential oils extracted from wood residues of Picea abies on the growth of Escherichia coli. The essential oils were extracted by supercritical carbon dioxide, leading to a yield of 3.4 ± 0.5% (w/w) in 120 min. The antimicrobial effect was tested at 37 °C by isothermal calorimetry. The heat-flow (dq/dt vs. time) was integrated to give a fractional reaction curve (α vs. time). Such curves were fitted by a modified Gompertz function to give the lag-time (λ) and the maximum growth rate (µmax) parameters. The results showed that λ was linearly correlated with E. coli concentration (λ = 1.4 h/log (CFU/mL), R2 = 0.997), whereas µmax was invariant. Moreover, the overall heat was nearly constant to all the dilutions of E. coli. Instead, when the essential oil was added (with concentrations ranging from 1 to 5 mg/L) to a culture of E. coli (104 CFU/mL), the lag-time increased from 14.1 to 33.7 h, and the overall heat decreased from 2120 to 2.37 J. The results obtained by the plate count technique were linear with the lag-time (λ), where (λ = −7.3 × log (CFU/mL) + 38.3, R2 = 0.9878). This suggested a lower capacity of E. coli to metabolize the substrate in the presence of the essential oils. The results obtained in this study promote the use of essential oils from wood residues and their use as antimicrobial products. View Full-Text
Keywords: Picea abies; supercritical fluid extraction; isothermal calorimetry; antimicrobials Picea abies; supercritical fluid extraction; isothermal calorimetry; antimicrobials
Show Figures

Figure 1

MDPI and ACS Style

Haman, N.; Morozova, K.; Tonon, G.; Scampicchio, M.; Ferrentino, G. Antimicrobial Effect of Picea abies Extracts on E. coli Growth. Molecules 2019, 24, 4053.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop