Next Article in Journal
Puccinellia maritima, Spartina maritime, and Spartina patens Halophytic Grasses: Characterization of Polyphenolic and Chlorophyll Profiles and Evaluation of Their Biological Activities
Previous Article in Journal
Simultaneous Determination and Quantification of Triterpene Saponins from Camellia sinensis Seeds Using UPLC-PDA-QTOF-MS/MS
Article

Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies

1
Faculty of Sciences, BioISI—Biosystems & Integrative Sciences Institute, University of Lisbon, 1649-004 Lisboa, Portugal
2
Faculty of Sciences, CQB—Centre of Chemistry and Biochemistry, University of Lisbon, 1649-004 Lisboa, Portugal
3
Área Departamental de Engenharia Química, Instituto Superior de Engenharia de Lisboa, Av. Conselheiro Emídio Navarro, 1959-007 Lisboa, Portugal
4
Faculty of Sciences, Departamento de Química e Bioquímica, University of Lisbon, Campo Grande, 1749-016 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(20), 3795; https://doi.org/10.3390/molecules24203795
Received: 17 September 2019 / Revised: 9 October 2019 / Accepted: 21 October 2019 / Published: 22 October 2019
Centaurium erythraea is recommended for the treatment of gastrointestinal disorders and to reduce hypercholesterolemia in ethno-medicinal practice. To perform a top-down study that could give some insight into the molecular basis of these bioactivities, decoctions from C. erythraea leaves were prepared and the compounds were identified by liquid chromatography-high resolution tandem mass spectrometry (LC–MS/MS). Secoiridoids glycosides, like gentiopicroside and sweroside, and several xanthones, such as di-hydroxy-dimethoxyxanthone, were identified. Following some of the bioactivities previously ascribed to C. erythraea, we have studied its antioxidant capacity and the ability to inhibit acetylcholinesterase (AChE) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR). Significant antioxidant activities were observed, following three assays: free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) reduction; lipoperoxidation; and NO radical scavenging capacity. The AChE and HMGR inhibitory activities for the decoction were also measured (56% at 500 μg/mL and 48% at 10 μg/mL, respectively). Molecular docking studies indicated that xanthones are better AChE inhibitors than gentiopicroside, while this compound exhibits a better shape complementarity with the HMGR active site than xanthones. To the extent of our knowledge, this is the first report on AChE and HMGR activities by C. erythraea decoctions, in a top-down analysis, complemented with in silico molecular docking, which aims to understand, at the molecular level, some of the biological effects ascribed to infusions from this plant. View Full-Text
Keywords: Centaurium erythraea; acetylcholinesterase; HMG-CoA reductase; gentiopicroside; xanthones; docking studies Centaurium erythraea; acetylcholinesterase; HMG-CoA reductase; gentiopicroside; xanthones; docking studies
Show Figures

Figure 1

MDPI and ACS Style

Guedes, L.; Reis, P.B.P.S.; Machuqueiro, M.; Ressaissi, A.; Pacheco, R.; Serralheiro, M.L. Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies. Molecules 2019, 24, 3795. https://doi.org/10.3390/molecules24203795

AMA Style

Guedes L, Reis PBPS, Machuqueiro M, Ressaissi A, Pacheco R, Serralheiro ML. Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies. Molecules. 2019; 24(20):3795. https://doi.org/10.3390/molecules24203795

Chicago/Turabian Style

Guedes, Laura; Reis, Pedro B.P.S.; Machuqueiro, Miguel; Ressaissi, Asma; Pacheco, Rita; Serralheiro, Maria L. 2019. "Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies" Molecules 24, no. 20: 3795. https://doi.org/10.3390/molecules24203795

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop