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Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

1
Department of Food Science & Human Nutrition, Agricultural University of Athens, 11855 Athens, Greece
2
Department of Food Science & Nutrition, School of the Environment, University of the Aegean, 81400 Myrina, Lemnos, Greece
*
Authors to whom correspondence should be addressed.
Academic Editor: Afroditi Chatzifragkou
Molecules 2019, 24(2), 222; https://doi.org/10.3390/molecules24020222
Received: 26 October 2018 / Revised: 21 December 2018 / Accepted: 31 December 2018 / Published: 9 January 2019
(This article belongs to the Special Issue Chemicals from Food Supply Chain By-Products and Waste Streams)
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Abstract

Olive mill wastewaters (OMW) are the major effluent deriving from olive oil production and are considered as one of the most challenging agro-industrial wastes to treat. Crude glycerol is the main by-product of alcoholic beverage and oleochemical production activities including biodiesel production. The tremendous quantities of glycerol produced worldwide represent a serious environmental challenge. The aim of this study was to assess the ability of Yarrowia lipolytica strain ACA-DC 5029 to grow on nitrogen-limited submerged shake-flask cultures, in crude glycerol and OMW blends as well as in media with high initial glycerol concentration and produce biomass, cellular lipids, citric acid and polyols. The rationale of using such blends was the dilution of concentrated glycerol by OMW to (partially or fully) replace process tap water with a wastewater stream. The strain presented satisfactory growth in blends; citric acid production was not affected by OMW addition (Citmax~37.0 g/L, YCit/Glol~0.55 g/g) and microbial oil accumulation raised proportionally to OMW addition (Lmax~2.0 g/L, YL/X~20% w/w). Partial removal of color (~30%) and phenolic compounds (~10% w/w) of the blended media occurred. In media with high glycerol concentration, a shift towards erythritol production was noted (Erymax~66.0 g/L, YEry/Glol~0.39 g/g) simultaneously with high amounts of produced citric acid (Citmax~79.0 g/L, YCit/Glol~0.46 g/g). Fatty acid analysis of microbial lipids demonstrated that OMW addition in blended media and in excess carbon media with high glycerol concentration favored oleic acid production. View Full-Text
Keywords: olive mill wastewaters; Yarrowia lipolytica; polyols; cellular lipids; citric acid; crude glycerol olive mill wastewaters; Yarrowia lipolytica; polyols; cellular lipids; citric acid; crude glycerol
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Sarris, D.; Rapti, A.; Papafotis, N.; Koutinas, A.A.; Papanikolaou, S. Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain. Molecules 2019, 24, 222.

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