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Open AccessArticle

Identification of Resveratrol as Bioactive Compound of Propolis from Western Romania and Characterization of Phenolic Profile and Antioxidant Activity of Ethanolic Extracts

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Doctoral School Medicine-Pharmacy, “Victor Babeş” University of Medicine and Pharmacy of Timişoara, Eftimie Murgu Sq. No. 2, 300041 Timişoara, Romania
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Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy of Timişoara, Eftimie Murgu Sq. No. 2, 300041 Timişoara, Romania
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Center for Translational Research & Systems Medicine, “Victor Babeş” University of Medicine and Pharmacy of Timişoara, Eftimie Murgu Sq. No. 2, 300041 Timişoara, Romania
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Faculty of Pharmacy, “Victor Babeş”, University of Medicine and Pharmacy of Timişoara, Eftimie Murgu Sq., No. 2, 300041 Timişoara, Romania
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Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”of Timişoara, Calea Aradului, No. 119, 300645 Timișoara, Romania
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Authors to whom correspondence should be addressed.
Academic Editor: Juraj Majtan
Molecules 2019, 24(18), 3368; https://doi.org/10.3390/molecules24183368
Received: 7 July 2019 / Revised: 8 September 2019 / Accepted: 11 September 2019 / Published: 16 September 2019
(This article belongs to the Special Issue Biological Activities of Honeybee Products)
The present study aimed to assess the phenolic content of eight ethanolic propolis samples (P1–P8) harvested from different regions of Western Romania and their antioxidant activity. The mean value of total phenolic content was 214 ± 48 mg gallic acid equivalents (GAE)/g propolis. All extracts contained kaempferol (514.02 ± 114.80 μg/mL), quercetin (124.64 ± 95.86 μg/mL), rosmarinic acid (58.03 ± 20.08 μg/mL), and resveratrol (48.59 ± 59.52 μg/mL) assessed by LC-MS. The antioxidant activity was evaluated using 2 methods: (i) DPPH (2,2-diphenyl-1-picrylhydrazyl) assay using ascorbic acid as standard antioxidant and (ii) FOX (Ferrous iron xylenol orange OXidation) assay using catalase as hydrogen peroxide (H2O2) scavenger. The DPPH radical scavenging activity was determined for all samples applied in 6 concentrations (10, 5, 3, 1.5, 0.5 and 0.3 mg/mL). IC50 varied from 0.0700 to 0.9320 mg/mL (IC50 of ascorbic acid = 0.0757 mg/mL). The % of H2O2 inhibition in FOX assay was assessed for P1, P2, P3, P4 and P8 applied in 2 concentrations (5 and 0.5 mg/mL). A significant H2O2% inhibition was obtained for these samples for the lowest concentration. We firstly report the presence of resveratrol as bioactive compound in Western Romanian propolis. The principal component analysis revealed clustering of the propolis samples according to the polyphenolic profile similarity. View Full-Text
Keywords: Western Romanian propolis; polyphenols; resveratrol; Folin-Ciocȃlteu; LC-MS; DPPH method; FOX assay Western Romanian propolis; polyphenols; resveratrol; Folin-Ciocȃlteu; LC-MS; DPPH method; FOX assay
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MDPI and ACS Style

Duca, A.; Sturza, A.; Moacă, E.-A.; Negrea, M.; Lalescu, V.-D.; Lungeanu, D.; Dehelean, C.-A.; Muntean, D.-M.; Alexa, E. Identification of Resveratrol as Bioactive Compound of Propolis from Western Romania and Characterization of Phenolic Profile and Antioxidant Activity of Ethanolic Extracts. Molecules 2019, 24, 3368.

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