Next Article in Journal
Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans
Previous Article in Journal
Effects of Seasonal Variability on the Physicochemical, Biochemical, and Nutritional Composition of Western Peninsular Malaysia Gracilaria manilaensis
Previous Article in Special Issue
Intake of Molecular Hydrogen in Drinking Water Increases Membrane Transporters, p-Glycoprotein, and Multidrug Resistance-Associated Protein 2 without Affecting Xenobiotic-Metabolizing Enzymes in Rat Liver
Open AccessArticle

Characterizing Tyrosinase Modulators from the Roots of Angelica keiskei Using Tyrosinase Inhibition Assay and UPLC-MS/MS as the Combinatorial Novel Approach

1
School of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan
2
Department of Science Education, National Taipei University of Education, Taipei 10671, Taiwan
3
Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
4
Institute of Fisheries Science, National Taiwan University, Taipei 10617, Taiwan
5
Taichung District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Taichung 42081, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Ping-Chung Kuo
Molecules 2019, 24(18), 3297; https://doi.org/10.3390/molecules24183297
Received: 20 August 2019 / Revised: 7 September 2019 / Accepted: 8 September 2019 / Published: 10 September 2019
(This article belongs to the Special Issue Food and Drug Analysis)
In this study, an in vitro tyrosinase inhibition assay in combination with ultra performance liquid chromatography-orbitrap mass spectrometry (UPLC-orbitrap-MS) was developed for the rapid screening and identification of tyrosinase modulators from roots of Angelica keiskei. Of the 15 candidates considered, nine chalcones, xanthoangelols (1), B (2), D (3), E (4), G (5), H (6), 4-hydroxyderricin (7), xanthokeismin B (8) and (2E)-1-[4-hydroxy-2-(2-hydroxy-2-propanyl)-2,3-dihydro-1-benzofuran-7-yl]-3-(4-hydroxyphenyl)-2-propen-1-one (9), five coumarins, umbelliferone (10), selinidin (11), isopimpinellin (12), phellopterin (13) and xanthyletin (14), and one other compound, ashitabaol A (15), were distinguished between the test samples and the controls with statistical significance, and the structure of each compound was determined by comparing with in-house standards and the literature. Among these, six compounds, xanthoangelol (1), xanthoangelol D (3), xanthoangelol H (6), 4-hydroxyderricin (7), laserpitin (16) and isolaserpitin (17), were isolated from roots of A. keiskei. Of the compounds isolated, compounds 1, 7 and 16 were subjected to tyrosinase inhibitory assay, and the IC50 values were 15.87 ± 1.21, 60.14 ± 2.29 and >100 μM, respectively. The present study indicated that the combination of in vitro tyrosinase inhibition assay coupled with UPLC-MS/MS could be widely applied to the rapid screening of active substances from various natural resources. View Full-Text
Keywords: Angelica keiskei; tyrosinase; UPLC-MS/MS; chalcone; coumarins Angelica keiskei; tyrosinase; UPLC-MS/MS; chalcone; coumarins
Show Figures

Graphical abstract

MDPI and ACS Style

Lee, J.-H.; Mei, H.-C.; Kuo, I.-C.; Lee, T.-H.; Chen, Y.-H.; Lee, C.-K. Characterizing Tyrosinase Modulators from the Roots of Angelica keiskei Using Tyrosinase Inhibition Assay and UPLC-MS/MS as the Combinatorial Novel Approach. Molecules 2019, 24, 3297.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop