Next Article in Journal
Permeable Water-Resistant Heat Insulation Panel Based on Recycled Materials and Its Physical and Mechanical Properties
Previous Article in Journal
Characterizing Tyrosinase Modulators from the Roots of Angelica keiskei Using Tyrosinase Inhibition Assay and UPLC-MS/MS as the Combinatorial Novel Approach
Previous Article in Special Issue
Chemical Composition and Antioxidant Properties of Essential Oils from Peppermint, Native Spearmint and Scotch Spearmint
Open AccessArticle

Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans

1
Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil
2
Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, São Paulo, Brazil
3
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, D-69120 Heidelberg, Germany
4
Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Departamento de Química, Universidade de São Paulo, Ribeirão Preto 14049-900, São Paulo, Brazil
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(18), 3299; https://doi.org/10.3390/molecules24183299
Received: 7 August 2019 / Revised: 31 August 2019 / Accepted: 4 September 2019 / Published: 10 September 2019
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products 2019)
Uncaria tomentosa (Rubiaceae) has a recognized therapeutic potential against various diseases associated with oxidative stress. The aim of this research was to evaluate the antioxidant potential of an aqueous leaf extract (ALE) from U. tomentosa, and its major alkaloids mitraphylline and isomitraphylline. The antioxidant activity of ALE was investigated in vitro using standard assays (DPPH, ABTS and  FRAP), while the in vivo activity and mode of action were studied using Caenorhabditis elegans as a model organism. The purified alkaloids did not exhibit antioxidant effects in vivo. ALE reduced the accumulation of reactive oxygen species (ROS) in wild-type worms, and was able to rescue the worms from a lethal dose of the pro-oxidant juglone. The ALE treatment led to a decreased expression of the oxidative stress response related genes sod-3, gst-4, and hsp-16.2. The treatment of mutant worms lacking the DAF-16 transcription factor with ALE resulted in a significant reduction of ROS levels. Contrarily, the extract had a pro-oxidant effect in the worms lacking the SKN-1 transcription factor. Our results suggest that the antioxidant activity of ALE in C. elegans is independent of its alkaloid content, and that SKN-1 is required for ALE-mediated stress resistance. View Full-Text
Keywords: Uncaria tomentosa; Caenorhabditis elegans; antioxidants; oxidative stress; mitraphylline; isomitraphylline Uncaria tomentosa; Caenorhabditis elegans; antioxidants; oxidative stress; mitraphylline; isomitraphylline
Show Figures

Figure 1

MDPI and ACS Style

C. Azevedo, B.; Roxo, M.; C. Borges, M.; Peixoto, H.; Crevelin, E.J.; W. Bertoni, B.; H. T. Contini, S.; Lopes, A.A.; C. França, S.; S. Pereira, A.M.; Wink, M. Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 2019, 24, 3299.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop