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Open AccessArticle

LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections

1
Glycoscience and Glycotechnology Research Group, Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8568, Japan
2
Glycan & Life Science Integration Center (GaLSIC), Faculty of Science and Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(16), 2962; https://doi.org/10.3390/molecules24162962
Received: 27 June 2019 / Revised: 5 August 2019 / Accepted: 12 August 2019 / Published: 15 August 2019
For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues. To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections. Using this “tissue glycome mapping” approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice. We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data. This online tool, called “LM-GlycomeAtlas”, allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas. Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns. Taking advantage of its extensibility, this tool will continue to be updated with the expansion of deposited data. View Full-Text
Keywords: mouse tissue glycome mapping; formalin-fixed paraffin-embedded tissue section; laser microdissection; lectin microarray mouse tissue glycome mapping; formalin-fixed paraffin-embedded tissue section; laser microdissection; lectin microarray
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Nagai-Okatani, C.; Aoki-Kinoshita, K.F.; Kakuda, S.; Nagai, M.; Hagiwara, K.; Kiyohara, K.; Fujita, N.; Suzuki, Y.; Sato, T.; Angata, K.; Kuno, A. LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections. Molecules 2019, 24, 2962.

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