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Genome-Wide and Functional View of Proteolytic and Lipolytic Bacteria for Efficient Biogas Production through Enhanced Sewage Sludge Hydrolysis

1
Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
2
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Molecules 2019, 24(14), 2624; https://doi.org/10.3390/molecules24142624
Received: 27 June 2019 / Revised: 17 July 2019 / Accepted: 17 July 2019 / Published: 18 July 2019
(This article belongs to the Special Issue Advances in Conversion of Biomass and Waste to Chemicals and Fuels)
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Abstract

In this study, we used a multifaceted approach to select robust bioaugmentation candidates for enhancing biogas production and to demonstrate the usefulness of a genome-centric approach for strain selection for specific bioaugmentation purposes. We also investigated the influence of the isolation source of bacterial strains on their metabolic potential and their efficiency in enhancing anaerobic digestion. Whole genome sequencing, metabolic pathway reconstruction, and physiological analyses, including phenomics, of phylogenetically diverse strains, Rummeliibacillus sp. POC4, Ochrobactrum sp. POC9 (both isolated from sewage sludge) and Brevundimonas sp. LPMIX5 (isolated from an agricultural biogas plant) showed their diverse enzymatic activities, metabolic versatility and ability to survive under varied growth conditions. All tested strains display proteolytic, lipolytic, cellulolytic, amylolytic, and xylanolytic activities and are able to utilize a wide array of single carbon and energy sources, as well as more complex industrial by-products, such as dairy waste and molasses. The specific enzymatic activity expressed by the three strains studied was related to the type of substrate present in the original isolation source. Bioaugmentation with sewage sludge isolates–POC4 and POC9–was more effective for enhancing biogas production from sewage sludge (22% and 28%, respectively) than an approach based on LPMIX5 strain (biogas production boosted by 7%) that had been isolated from an agricultural biogas plant, where other type of substrate is used. View Full-Text
Keywords: sewage sludge hydrolysis; bioaugmentation; whole genome sequencing; metabolic pathway reconstruction; phenomic analysis; anaerobic digestion; biogas sewage sludge hydrolysis; bioaugmentation; whole genome sequencing; metabolic pathway reconstruction; phenomic analysis; anaerobic digestion; biogas
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Poszytek, K.; Karczewska-Golec, J.; Dziurzynski, M.; Stepkowska-Kowalska, O.; Gorecki, A.; Decewicz, P.; Dziewit, L.; Drewniak, L. Genome-Wide and Functional View of Proteolytic and Lipolytic Bacteria for Efficient Biogas Production through Enhanced Sewage Sludge Hydrolysis. Molecules 2019, 24, 2624.

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