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Open AccessArticle

Study on the Material Basis of Houpo Wenzhong Decoction by HPLC Fingerprint, UHPLC-ESI-LTQ-Orbitrap-MS, and Network Pharmacology

1
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Yangguang South Avenue, Fangshan District, Beijing 102488, China
2
Yifan Pharmaceutical Co., Ltd., Lin’an 311300, China
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(14), 2561; https://doi.org/10.3390/molecules24142561
Received: 30 June 2019 / Revised: 9 July 2019 / Accepted: 9 July 2019 / Published: 14 July 2019
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Abstract

Houpo Wenzhong Decoction (HWD) as a classical prescription has been widely used for hundreds of years. However, the quality of HWDs is difficult to control because of its herb materials from different regions. It is a new idea to use HPLC fingerprints, LTQ-ESI-Orbitrap-MS, and network pharmacology in combination to screen common components (CCs) as potential quality control indicators. In this paper, the fingerprints of HWDs were established, which were used to determine HWDs compounded from different sources of traditional Chinese medicines (TCMs). Through the similarity analysis, 45 CCs were selected. UHPLC-LTQ-ESI-Orbitrap-MS was used to carry out the chemical composition analysis of HWD. Seventy-three chemical constituents were distinguished, and 30 CCs were identified. Through network pharmacology, networks of candidate CCs, diseases, and candidate targets were constructed. Finally, four CCs were screened as potential active ingredient markers of HWD, and a method for content determination of these four components was established. A rapid, reasonable, and effective method for quality evaluation and control of HWDs was established. It provides a reference for the further development and research of HWDs and a new way of thinking for the research of other Chinese medicine prescriptions. View Full-Text
Keywords: Houpo Wenzhong decoction; UHPLC-ESI-LTQ-Orbitrap-MS; network pharmacology; material basis Houpo Wenzhong decoction; UHPLC-ESI-LTQ-Orbitrap-MS; network pharmacology; material basis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Luo, J.; Chen, G.; Liu, D.; Wang, Y.; Qi, Q.; Hu, H.; Li, P.; Bai, J.; Du, S.; Lu, Y.; Wang, Y.; Liu, C. Study on the Material Basis of Houpo Wenzhong Decoction by HPLC Fingerprint, UHPLC-ESI-LTQ-Orbitrap-MS, and Network Pharmacology. Molecules 2019, 24, 2561.

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