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Open AccessArticle

Comparison of the Phytochemical Composition of Serenoa repens Extracts by a Multiplexed Metabolomic Approach

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UMR 152 Pharma Dev, Université de Toulouse, IRD, UPS, 31400 Toulouse, France
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Institut de Recherche Pierre Fabre, Centre de R&D Pierre Fabre, Green Mission Pierre Fabre, 3 Avenue Hubert Curien, BP 13562, 31035 Toulouse CEDEX, France
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(12), 2208; https://doi.org/10.3390/molecules24122208
Received: 19 May 2019 / Revised: 6 June 2019 / Accepted: 11 June 2019 / Published: 13 June 2019
(This article belongs to the Section Natural Products Chemistry)
Phytochemical extracts are highly complex chemical mixtures. In the context of an increasing demand for phytopharmaceuticals, assessment of the phytochemical equivalence of extraction procedures is of utmost importance. Compared to routine analytical methods, comprehensive metabolite profiling has pushed forward the concept of phytochemical equivalence. In this study, an untargeted metabolomic approach was used to cross-compare four marketed extracts from Serenoa repens obtained with three different extraction processes: ethanolic, hexanic and sCO2 (supercritical carbon dioxide). Our approach involved a biphasic extraction of native compounds followed by liquid chromatography coupled to a high-resolution mass spectrometry based metabolomic workflow. Our results showed significant differences in the contents of major and minor compounds according to the extraction solvent used. The analyses showed that ethanolic extracts were supplemented in phosphoglycerides and polyphenols, hexanic extracts had higher amounts of free fatty acids and minor compounds, and sCO2 samples contained more glycerides. The discriminant model in this study could predict the extraction solvent used in commercial samples and highlighted the specific biomarkers of each process. This metabolomic survey allowed the authors to assess the phytochemical content of extracts and finished products of S. repens and unequivocally established that sCO2, hexanic and ethanolic extracts are not chemically equivalent and are therefore unlikely to be pharmacologically equivalent. View Full-Text
Keywords: metabolomics; phytochemical equivalence; Serenoa repens extract; Saw palmetto; natural products metabolomics; phytochemical equivalence; Serenoa repens extract; Saw palmetto; natural products
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MDPI and ACS Style

Marti, G.; Joulia, P.; Amiel, A.; Fabre, B.; David, B.; Fabre, N.; Fiorini-Puybaret, C. Comparison of the Phytochemical Composition of Serenoa repens Extracts by a Multiplexed Metabolomic Approach. Molecules 2019, 24, 2208.

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