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Antidiabetic Activity and Potential Mechanism of Amentoflavone in Diabetic Mice

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Mr. and Mrs. Ko Chi Ming centre for Parkinson’s Disease Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
Authors to whom correspondence should be addressed.
Molecules 2019, 24(11), 2184;
Received: 22 May 2019 / Revised: 4 June 2019 / Accepted: 10 June 2019 / Published: 11 June 2019
Aim: To investigate the anti-diabetic activity of amentoflavone (AME) in diabetic mice, and to explore the potential mechanisms. Methods: Diabetic mice induced by high fat diet and streptozotocin were administered with amentoflavone for 8 weeks. Biochemical indexes were tested to evaluate its anti-diabetic effect. Hepatic steatosis, the histopathology change of the pancreas was evaluated. The activity of glucose metabolic enzymes, the expression of Akt and pAkt, and the glucose transporter type 4 (GLUT4) immunoreactivity were detected. Results: AME decreased the level of glucose, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and glucagon, and increased the levels of high density lipoprotein cholesterol (HDL-C) and insulin. Additionally, AME increased the activity of glucokinase (GCK), phosphofructokinase-1 (PFK-1), and pyruvate kinase (PK), and inhibited the activity of glycogen synthase kinase-3 (GSK-3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G-6-Pase). Mechanistically, AME increased superoxide dismutase (SOD), decreased malondialdehyde (MDA), activation of several key signaling molecules including pAkt (Ser473), and increased the translocation to the sedimenting membranes of GLUT4 in skeletal muscle tissue. Conclusions: AME exerted anti-diabetic effects by regulating glucose and lipid metabolism, perhaps via anti-oxidant effects and activating the PI3K/Akt pathway. Our study provided novel insight into the role and underlying mechanisms of AME in diabetes. View Full-Text
Keywords: biochemical indexes; glucose metabolic enzyme; PI3K/Akt; GLUT4 translocation biochemical indexes; glucose metabolic enzyme; PI3K/Akt; GLUT4 translocation
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Su, C.; Yang, C.; Gong, M.; Ke, Y.; Yuan, P.; Wang, X.; Li, M.; Zheng, X.; Feng, W. Antidiabetic Activity and Potential Mechanism of Amentoflavone in Diabetic Mice. Molecules 2019, 24, 2184.

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