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Open AccessArticle

Interactions between β-Lactoglobulin and 3,3′-Diindolylmethane in Model System

1
Key Laboratory of Dairy Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
2
Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun 130062, Jilin, China
3
Department of Nutrition and Food Sciences, College of Agriculture and Life Sciences, University of Vermont, Burlington, VT 05405, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Yangchao Luo
Molecules 2019, 24(11), 2151; https://doi.org/10.3390/molecules24112151
Received: 8 May 2019 / Revised: 31 May 2019 / Accepted: 6 June 2019 / Published: 7 June 2019
The compound 3,3′-diindolylmethane (DIM) has a broad spectrum of anticancer activities. However, low stability and bioavailability limit its application. Elucidating interactions between DIM and β-lactoglobulin (β-LG) may be useful for fabricating whey protein-based protecting systems. Interaction with DIM increased the diameter and absolute zeta potential value of β-LG. UV-absorption spectra suggested that there was a complex of DIM and β-LG. β-LG showed enhanced fluorescence intensity by complexing with DIM with a binding constant of 6.7 × 105 M−1. Upon interaction with DIM, β-LG was decreased in secondary structure content of helix and turn while increased in β-sheet and unordered. FT-IR spectra and molecular docking results indicated the roles of hydrophobic interaction and hydrogen bond for the formation of DIM and β-LG nanocomplexes. Data suggested that β-LG may be a good vehicle for making a protein-based DIM protection and delivery system due to the tight binding of DIM to β-LG. View Full-Text
Keywords: 3,3′-diindolylmethane; β-lactoglobulin; spectroscopic analysis; molecular docking 3,3′-diindolylmethane; β-lactoglobulin; spectroscopic analysis; molecular docking
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MDPI and ACS Style

Wang, C.; Zhou, X.; Wang, H.; Sun, X.; Guo, M. Interactions between β-Lactoglobulin and 3,3′-Diindolylmethane in Model System. Molecules 2019, 24, 2151.

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