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Open AccessArticle

Applying Nanoparticle Tracking Analysis to Characterize the Polydispersity of Aggregates Resulting from Tannin–Polysaccharide Interactions in Wine-Like Media

1
School of Agriculture, Food and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia
2
The Australian Research Council Training Centre for Innovative Wine Production, PMB 1, Glen Osmond, SA 5064, Australia
3
The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA 5064, Australia
*
Author to whom correspondence should be addressed.
Current address: National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
Academic Editors: Rosa Maria de Sá Perestrelo and José Sousa Câmara
Molecules 2019, 24(11), 2100; https://doi.org/10.3390/molecules24112100
Received: 26 April 2019 / Revised: 28 May 2019 / Accepted: 29 May 2019 / Published: 3 June 2019
(This article belongs to the Special Issue Chemical/Instrumental Approaches to the Evaluation of Wine Chemistry)
Interactions between grape seed tannin and either a mannoprotein or an arabinogalactan in model wine solutions of different ethanol concentrations were characterized with nanoparticle tracking analysis (NTA), UV-visible spectroscopy and dynamic light scattering (DLS). NTA results reflected a shift in particle size distribution due to aggregation. Furthermore, the light scattering intensity of each tracked particle measured by NTA demonstrated the presence of aggregates, even when a shift in particle size was not apparent. Mannoprotein and arabinogalactan behaved differently when combined with seed tannin. Mannoprotein formed large, highly light-scattering aggregates, while arabinogalactan exhibited only weak interactions with seed tannin. A 3% difference in alcohol concentration of the model solution (12 vs. 15% v/v) was sufficient to affect the interactions between mannoprotein and tannin when the tannin concentration was high. In summary, this study showed that NTA is a promising tool for measuring polydisperse samples of grape and wine macromolecules, and their aggregates under wine-like conditions. The implications for wine colloidal properties are discussed based on these results. View Full-Text
Keywords: mannoprotein; arabinogalactan; seed tannin; aggregation; nanoparticle tracking analysis mannoprotein; arabinogalactan; seed tannin; aggregation; nanoparticle tracking analysis
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MDPI and ACS Style

Li, S.; Wilkinson, K.L.; Mierczynska-Vasilev, A.; Bindon, K.A. Applying Nanoparticle Tracking Analysis to Characterize the Polydispersity of Aggregates Resulting from Tannin–Polysaccharide Interactions in Wine-Like Media. Molecules 2019, 24, 2100. https://doi.org/10.3390/molecules24112100

AMA Style

Li S, Wilkinson KL, Mierczynska-Vasilev A, Bindon KA. Applying Nanoparticle Tracking Analysis to Characterize the Polydispersity of Aggregates Resulting from Tannin–Polysaccharide Interactions in Wine-Like Media. Molecules. 2019; 24(11):2100. https://doi.org/10.3390/molecules24112100

Chicago/Turabian Style

Li, Sijing; Wilkinson, Kerry L.; Mierczynska-Vasilev, Agnieszka; Bindon, Keren A. 2019. "Applying Nanoparticle Tracking Analysis to Characterize the Polydispersity of Aggregates Resulting from Tannin–Polysaccharide Interactions in Wine-Like Media" Molecules 24, no. 11: 2100. https://doi.org/10.3390/molecules24112100

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