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Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays

1
Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea
2
Division of Bio-Medical Science and Technology (Biological Chemistry), Korea University of Science and Technology (UST), Daejeon 34113, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Angela R. Piergiovanni and José Manuel Herrero-Martínez
Molecules 2019, 24(10), 1977; https://doi.org/10.3390/molecules24101977
Received: 24 April 2019 / Revised: 20 May 2019 / Accepted: 21 May 2019 / Published: 22 May 2019
(This article belongs to the Special Issue Analysis of Peptides and Proteins by Electrophoretic Techniques)
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Abstract

Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods. The use of CE-LIF in immunoassays and enzyme assays has gained a reputation in recent years for its high detection sensitivity, short analysis time, and accurate quantification. Immunoassays are bioassay platforms that rely on binding reactions between an antigen (analyte) and a specific antibody. Enzyme assays measure enzymatic activity through quantitative analysis of substrates and products by the reaction of enzymes in purified enzyme or cell systems. These two category analyses play an important role in the context of biopharmaceutical analysis, clinical therapy, drug discovery, and diagnosis analysis. This review discusses the expanding portfolio of immune and enzyme assays using CE-LIF and focuses on the advantages and disadvantages of these methods over the ten years of existing technology since 2008. View Full-Text
Keywords: CE-LIF; immunoassay; enzyme assay; chip-based CE-LIF assay CE-LIF; immunoassay; enzyme assay; chip-based CE-LIF assay
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Nguyen, B.T.; Kang, M.-J. Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays. Molecules 2019, 24, 1977.

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