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Molecules 2018, 23(9), 2283;

Sol-Gel Immobilisation of Lipases: Towards Active and Stable Biocatalysts for the Esterification of Valeric Acid

Organic Chemistry Department, University of Cordoba, Campus de Rabanales, Edificio Marie Curie (C-3), Ctra. Nacional IV-A, Km 396, E14014 Cordoba, Spain
Scientific Centre for Molecular Design and Synthesis of Innovative Compounds for Medicine, Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya str., 117198 Moscow, Russia
Author to whom correspondence should be addressed.
Received: 31 July 2018 / Revised: 1 September 2018 / Accepted: 4 September 2018 / Published: 6 September 2018
(This article belongs to the Special Issue Sol-Gel Chemistry. From Molecule to Functional Materials)
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Alkyl esters are high added value products useful in a wide range of industrial sectors. A methodology based on a simple sol-gel approach (biosilicification) is herein proposed to encapsulate enzymes in order to design highly active and stable biocatalysts. Their performance was assessed through the optimization of valeric acid esterification evaluating the effect of different parameters (biocatalyst load, presence of water, reaction temperature and stirring rate) in different alcoholic media, and comparing two different methodologies: conventional heating and microwave irradiation. Ethyl valerate yields were in the 80–85% range under optimum conditions (15 min, 12% m/v biocatalyst, molar ratio 1:2 of valeric acid to alcohol). Comparatively, the biocatalysts were slightly deactivated under microwave irradiation due to enzyme denaturalisation. Biocatalyst reuse was attempted to prove that good reusability of these sol-gel immobilised enzymes could be achieved under conventional heating. View Full-Text
Keywords: sol-gel biosilicification; esterification; conventional heating; microwaves sol-gel biosilicification; esterification; conventional heating; microwaves

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Cebrián-García, S.; Balu, A.M.; García, A.; Luque, R. Sol-Gel Immobilisation of Lipases: Towards Active and Stable Biocatalysts for the Esterification of Valeric Acid. Molecules 2018, 23, 2283.

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