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Molecules 2018, 23(9), 2274; https://doi.org/10.3390/molecules23092274

Application of Centrifugal Partition Chromatography for Bioactivity-Guided Purification of Antioxidant-Response-Element-Inducing Constituents from Atractylodis Rhizoma Alba

1
College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Korea
2
Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamshoro 76080, Pakistan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 3 August 2018 / Revised: 3 September 2018 / Accepted: 5 September 2018 / Published: 6 September 2018
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Activity-guided separation of antioxidant response element (ARE)-inducing constituents from the rhizomes of Atractylodis Rhizoma Alba was performed by the combination of centrifugal partition chromatography (CPC) and an ARE luciferase reporter assay. From 3 g of the active n-hexane fraction, one polyacetylene, (6E,12E)-tetradeca-6,12-dien-8,10-diyne-1,3-diyl diacetate (47.3 mg), and two sesquiterpenes, atractylenolide I (40.9 mg), and selina-4(14),7(11)-dien-8-one (6.0 mg) were successfully isolated by CPC with n-hexane–ethyl acetate–methanol–water (8:2:8:2, v/v). The chemical structures of the isolated compounds were determined by 1H- and 13C-NMR and ESI-MS. Among the isolated compounds, (6E,12E)-tetradeca-6,12-diene-8,10-diyne-1,3-diol diacetate and selina-4(14),7(11)-dien-8-one increased ARE activity 32.9-fold and 16.6-fold, respectively, without significant cytotoxicity, when 5 µM sulforaphane enhanced ARE activity 27.1-fold. However, atractylenolide I did not increase ARE activity at 100 µM, and showed cytotoxicity at concentrations over 10 µM. View Full-Text
Keywords: Atractylodis Rhizoma Alba; centrifugal partition chromatography; antioxidant response element; polyacetylene; sesquiterpenes Atractylodis Rhizoma Alba; centrifugal partition chromatography; antioxidant response element; polyacetylene; sesquiterpenes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kim, M.I.; Kim, J.H.; Syed, A.S.; Kim, Y.-M.; Choe, K.K.; Kim, C.Y. Application of Centrifugal Partition Chromatography for Bioactivity-Guided Purification of Antioxidant-Response-Element-Inducing Constituents from Atractylodis Rhizoma Alba. Molecules 2018, 23, 2274.

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