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Genome-Wide Identification and Characterization of Aquaporins and Their Role in the Flower Opening Processes in Carnation (Dianthus caryophyllus)

by 1,2,3, 1,2,4 and 1,2,*
1
Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
2
Key Laboratory of Urban Agriculture in Central China (pilot run), Ministry of Agriculture, Wuhan 430070, China
3
State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China
4
Laboratoire Reproduction et Development des Plantes, Ecole Normale Supérieure Lyon, 520074 Lyon, France
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(8), 1895; https://doi.org/10.3390/molecules23081895
Received: 5 July 2018 / Revised: 23 July 2018 / Accepted: 25 July 2018 / Published: 29 July 2018
Aquaporins (AQPs) are associated with the transport of water and other small solutes across biological membranes. Genome-wide identification and characterization will pave the way for further insights into the AQPs’ roles in the commercial carnation (Dianthus caryophyllus). This study focuses on the analysis of AQPs in carnation (DcaAQPs) involved in flower opening processes. Thirty DcaAQPs were identified and grouped to five subfamilies: nine PIPs, 11 TIPs, six NIPs, three SIPs, and one XIP. Subsequently, gene structure, protein motifs, and co-expression network of DcaAQPs were analyzed and substrate specificity of DcaAQPs was predicted. qRT-PCR, RNA-seq, and semi-qRTRCR were used for DcaAQP genes expression analysis. The analysis results indicated that DcaAQPs were relatively conserved in gene structure and protein motifs, that DcaAQPs had significant differences in substrate specificity among different subfamilies, and that DcaAQP genes’ expressions were significantly different in roots, stems, leaves and flowers. Five DcaAQP genes (DcaPIP1;3, DcaPIP2;2, DcaPIP2;5, DcaTIP1;4, and DcaTIP2;2) might play important roles in flower opening process. However, the roles they play are different in flower organs, namely, sepals, petals, stamens, and pistils. Overall, this study provides a theoretical basis for further functional analysis of DcaAQPs. View Full-Text
Keywords: AQP; carnation; qRT-PCR; RNA-seq; co-expression network; flower opening process AQP; carnation; qRT-PCR; RNA-seq; co-expression network; flower opening process
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Kong, W.; Bendahmane, M.; Fu, X. Genome-Wide Identification and Characterization of Aquaporins and Their Role in the Flower Opening Processes in Carnation (Dianthus caryophyllus). Molecules 2018, 23, 1895.

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