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Molecules 2018, 23(7), 1813; https://doi.org/10.3390/molecules23071813

Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes

1
Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, Tandogan, TR-06100 Ankara, Turkey
2
Department of Pharmaceutical Sciences, University of Padua, Via Marzolo 5, I-35100 Padova, Italy
3
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1946/1, CZ-61242 Brno, Czech Republic
4
Department of Biology, Faculty of Art and Science, Yüzüncü Yıl University, TR-65080 Van, Turkey
*
Authors to whom correspondence should be addressed.
Academic Editor: Maria Carla Marcotullio
Received: 22 May 2018 / Revised: 14 July 2018 / Accepted: 16 July 2018 / Published: 21 July 2018
(This article belongs to the Special Issue Qualitative and Quantitative Analysis of Bioactive Natural Products)
Full-Text   |   PDF [1312 KB, uploaded 21 July 2018]   |  

Abstract

Previously tested n-hexane extracts of the Scorzonera latifolia showed promising bioactivity in vivo. Because triterpenes could account for this activity, n-hexane extracts were analyzed by HPLC to identify and quantify the triterpenes as the most abundant constituents. Other Scorzonera and Podospermum species, potentially containing triterpenic aglycones, were included in the study. An HPLC method for simultaneous determination of triterpene aglycones was therefore developed for analysis of Podospermum and Scorzonera species. n-Hexane extracts of root and aerial parts of S. latifolia, ten other Scorzonera species and two Podospermum species were studied to compare the content of triterpenes. HPLC was used for the qualitative and quantitative analysis of α-amyrin, lupeol, lupeol acetate, taraxasteryl acetate, 3-β-hydroxy-fern-7-en-6-one acetate, urs-12-en-11-one-3-acetyl, 3-β-hydroxy-fern-8-en-7-one acetate, and olean-12-en-11-one-3-acetyl. Limits of detection and quantification were determined for each compound. HPLC fingerprinting of n-hexane extracts of Podospermum and Scorzonera species revealed relatively large amounts of triterpenes in a majority of investigated taxa. Lupeol, lupeol acetate, and taraxasteryl acetate were found in a majority of the species, except S. acuminata. The presence of α-amyrin, 3β-hydroxy-fern-7-en-6-one-acetate, urs-12-en-11-one-3-acetyl, 3β-hydroxy-fern-8-en-7-one-acetate, and olean-12-en-11-one-3-acetyl was detected in varying amounts. The triterpene content could correlate with the analgesic and anti-inflammatory activity of Scorzonera, which was previously observed and Scorzonera species that have been determined to contain triterpenes in large amounts and have not yet been tested for their analgesic activity should be tested for their potential analgesic and anti-inflammatory potential. The presented HPLC method can be used for analysis of triterpene aglycones, for example dedicated to chemosystematic studies of the Scorzonerinae. View Full-Text
Keywords: HPLC; Podospermum; Scorzonera; triterpenes HPLC; Podospermum; Scorzonera; triterpenes
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Bahadır-Acıkara, Ö.; Özbilgin, S.; Saltan-İşcan, G.; Dall’Acqua, S.; Rjašková, V.; Özgökçe, F.; Suchý, V.; Šmejkal, K. Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes. Molecules 2018, 23, 1813.

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