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Molecules 2018, 23(7), 1650;

Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS

School of Pharmaceutical Sciences, Jilin University, Fujin Road 1266, Changchun 130021, China
Department of Pathogenic Biology, Basic Medical College, Jilin University, Xinmin Street 126, Changchun 130021, China
Authors to whom correspondence should be addressed.
Received: 6 June 2018 / Revised: 3 July 2018 / Accepted: 3 July 2018 / Published: 6 July 2018
(This article belongs to the Section Analytical Chemistry)
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Blood stasis syndrome (BSS) is one of the most common Chinese medicine patterns in coronary heart disease. Our previous work proved that Xueshuan Xinmaining Tablet (XXT) could treat blood stasis through regulating the expression of F13a1, Car1 and Tbxa2r. In the current study, the effect and mechanism of XXT on BSS was comprehensively and holistically investigated based on a metabolomics approach. Urine and plasma samples of 10 BBS rats treated with XXT (XT), 9 BSS model rats (BM) and 11 normal control (NC) rats were collected and then determined by UPLC-Q/TOP-MS. Multivariate analyses were applied to distinguish differentiate urinary and plasma metabolite patterns between three groups. Results showed that a clear separation of three groups was achieved. XT group was located between BM group and NC group, and showing a tendency of recovering to NC group, which was consistent with the results of hemorheological studies. Some significantly changed metabolites like cortexolone, 3α,21-dihydroxy-5β-pregnane-11,20-dione and 19S-hete and leukotriene A4, chiefly involved in steroid hormone biosynthesis, arachidonic acid metabolism and lipid metabolism, were found and identified to explain the mechanism. These potential markers and their corresponding pathways will help explain the mechanism of BSS and XXT treatment. This work also proves that metabolomics is effective in traditional Chinese medicinal research. View Full-Text
Keywords: blood stasis syndrome; Xueshuan Xinmaining tablet; UPLC-Q/TOF-MS; rats; metabolomics blood stasis syndrome; Xueshuan Xinmaining tablet; UPLC-Q/TOF-MS; rats; metabolomics

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Tan, J.; Wang, C.; Zhu, H.; Zhou, B.; Xiong, L.; Wang, F.; Li, P.; Liu, J. Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS. Molecules 2018, 23, 1650.

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