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Molecules 2018, 23(7), 1535; https://doi.org/10.3390/molecules23071535

Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma

1
Department of Biopharmacy, University of Debrecen, 4032 Debrecen, Hungary
2
Department of Pathology, University of Debrecen, 4032 Debrecen, Hungary
3
Endocrine, Polypeptide and Cancer Institute, Veterans Affairs Medical Center, Miami, FL 33125, USA
4
Department of Pathology, Miller School of Medicine, University of Miami, Miami, FL 33101, USA
5
Department of Medicine, Divisions of Hematology-Oncology and Endocrinology, Miller School of Medicine, University of Miami, Miami, FL 33101, USA
6
Sylvester Comprehensive Cancer Center, University of Miami, Miami, FL 33136, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Simona Collina and Mariarosaria Miloso
Received: 29 May 2018 / Revised: 20 June 2018 / Accepted: 22 June 2018 / Published: 26 June 2018
(This article belongs to the Special Issue Recent Advances in Anticancer Drugs)
Full-Text   |   PDF [3558 KB, uploaded 26 June 2018]   |  

Abstract

Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors. View Full-Text
Keywords: human uveal melanoma; somatostatin receptors; molecular targets SSTR-1-5; cancer therapy human uveal melanoma; somatostatin receptors; molecular targets SSTR-1-5; cancer therapy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Harda, K.; Szabo, Z.; Szabo, E.; Olah, G.; Fodor, K.; Szasz, C.; Mehes, G.; Schally, A.V.; Halmos, G. Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma. Molecules 2018, 23, 1535.

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