Ponasterone A and F, Ecdysteroids from the Arctic Bryozoan Alcyonidium gelatinosum
Abstract
:1. Introduction
2. Results and Discussion
2.1. Biomass Collection, Extraction and Fractionation
2.2. Viability Screening of the Flash Fractions
2.3. Dereplication of the Active Fraction
2.4. Isolation of 1 and 2
2.5. Structure Elucidation of Compound 1
2.6. Estrogen Receptor Agonist Activities of 1 and 2
2.7. Antiproliferative and Antibacterial Properties of 1 and 2
2.8. Probable Natural Functions of 1 and 2 in A. gelatinosum
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Biological Material
3.3. Extraction and Fractionation
3.4. Cytotoxicity Screening (MTS Assay) of the Flash Fractions
3.5. Dereplication of the Active Fraction
3.6. Isolation of 1 and 2
3.7. Agonistic Activity of 1 and 2 against Estrogen Receptors Alpha and Beta
3.8. Antiproliferative and Antibacterial Properties of 1 and 2
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available. The extract of A. gelatinosum is stored at the Norwegian national biobank (Marbank) under ID M14055. |
Ponasterone F (1) | |||
---|---|---|---|
Position | δC, Type | δH (J in Hz) | δOH (J in Hz) |
1 | 36.6, CH2 | 1.25, dd, 13.3, 11.9/1.58, dd, 13.3, 4.3 | |
2 | 66.7, CH | 3.64, dt, 11.7, 3.3 | |
3 | 66.7, CH | 3.73, d, 3.8 | 4.35 |
4 | 31.8, CH2 | 1.49, m 1,2 | 4.37 |
5 | 50.1, CH | 2.19, dd, 11.9, 5.3 | |
6 | 201.9, C | ||
7 | 120.7, CH | 5.45, s | |
8 | 164.9, C | ||
9 | 37.5, CH | 2.59, t, 7.7 | |
10 | 37.3, C | ||
11 | 21.4, CH2 | 1.75, ddt, 13.4, 6.6, 3.1/1.59, m 1,2 | |
12 | 38.7, CH2 | 2.15, m 1,2/1.49, m 1,2 | |
13 | 45.0, C | ||
14 | 55.0, CH | 2.13, m 1,2 | |
15 | 22.01, CH2 | 1.55, m 1,2/1.44, m 1,2 | |
16 | 21.3, CH2 | 1.89, m 2/1.51, m 1,2 | |
17 | 54.4, CH | 1.66, t, 9.4 | |
18 | 14.0, CH3 | 0.71, s | |
19 | 24.1, CH3 | 0.83, s | |
20 | 75.4, C | 3.62 | |
21 | 20.8, CH3 | 1.08, s | |
22 | 75.5, CH | 3.12, dd, 10.0, 1.7 | 4.36 |
23 | 29.0, CH2 | 1.38, m 1,2/1.09, m 1,2 | |
24 | 36.1, CH2 | 1.37, m 1,2/1.13, m 1,2 | |
25 | 27.4, CH | 1.50, m 1,2 | |
26 | 23.0, CH3 | 0.86, d, 6.6 | |
27 | 22.3, CH3 | 0.85, d, 6.6 |
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Hansen, K.Ø.; Isaksson, J.; Glomsaker, E.; Andersen, J.H.; Hansen, E. Ponasterone A and F, Ecdysteroids from the Arctic Bryozoan Alcyonidium gelatinosum. Molecules 2018, 23, 1481. https://doi.org/10.3390/molecules23061481
Hansen KØ, Isaksson J, Glomsaker E, Andersen JH, Hansen E. Ponasterone A and F, Ecdysteroids from the Arctic Bryozoan Alcyonidium gelatinosum. Molecules. 2018; 23(6):1481. https://doi.org/10.3390/molecules23061481
Chicago/Turabian StyleHansen, Kine Østnes, Johan Isaksson, Eirin Glomsaker, Jeanette Hammer Andersen, and Espen Hansen. 2018. "Ponasterone A and F, Ecdysteroids from the Arctic Bryozoan Alcyonidium gelatinosum" Molecules 23, no. 6: 1481. https://doi.org/10.3390/molecules23061481
APA StyleHansen, K. Ø., Isaksson, J., Glomsaker, E., Andersen, J. H., & Hansen, E. (2018). Ponasterone A and F, Ecdysteroids from the Arctic Bryozoan Alcyonidium gelatinosum. Molecules, 23(6), 1481. https://doi.org/10.3390/molecules23061481