Comparison of Characteristics and DNAzyme Activity of G4–Hemin Conjugates Obtained via Two Hemin Attachment Methods
Abstract
:1. Introduction
2. Results
2.1. Chemistry
2.2. Spectroscopic Characterization
2.3. Activity of DNAzymes
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Circular Dichroism and Thermal Denaturation Experiments
4.3. Spectrophotometric Determination of Hemin pKa
4.4. Peroxidase Activity Measurements
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Not Available. |
Name | Sequence |
---|---|
PS2.M | 5′–GTG GGT AGG GCG GGT TGG–3′ |
CatG4 | 5′–TGG GTA GGG CGG GTT GGG AAA–3′ |
PS2.M–hem1 | Hemin–(C6H12)–5′–GTG GGT AGG GCG GGT TGG–3′ |
PS2.M–hem2 | Hemin–(C35N5O5H49)–5′–GTG GGT AGG GCG GGT TGG–3′ |
CatG4–hem1 | Hemin–(C6H12)–5′–TGG GTA GGG CGG GTT GGG AAA–3′ |
CatG4–hem2 | Hemin–(C35N5O5H49)–5′–TGG GTA GGG CGG GTT GGG AAA–3′ |
Sequence | Tm [°C], 100 mM KCl | Tm [°C], 100 mM NaCl |
---|---|---|
PS2.M | 56.5 ± 0.9 | 46.0 ± 0.4 |
PS2.M/hemin | 64.6 ± 1.0 | 45.2 ± 0.6 |
PS2.M–hem1 | >80.0 a | >80.0 a |
PS2.M–hem2 | >80.0 a | 58.4 ± 1.0 |
CatG4 | 79.2 ± 1.5 | 41.8 ± 0.5 |
CatG4/hemin | >80.0 a | 42.2 ± 1.4 |
CatG4–hem2 | >80.0 a | 53.0 ± 1.9 |
CatG4–hem1 | >80.0 a | >80.0 a |
>80.0 a,b | >80.0 a,b | |
>80.0 a,c | 79.6 ± 0.3 c | |
85.2 ± 1.2 d | 73.0 ± 0.4 d | |
64.0 ± 0.7 e | 64.0 ± 0.7 e |
Sequence | K+ | Na+ |
---|---|---|
PS2.M/hemin | >9 1 | 6.9 ± 0.2 |
PS2.M–hem1 | 9.2 ± 0.1 | 9.1 ± 0.2 |
PS2.M–hem2 | 8.7 ± 0.1 | Nd |
CatG4/hemin | >9 1 | 8.5 ± 0.2 |
CatG4–hem1 | 8.8 ± 0.2 | 8.6 ± 0.3 |
CatG4–hem2 | 8.7 ± 0.3 | Nd |
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Kosman, J.; Żukowski, K.; Juskowiak, B. Comparison of Characteristics and DNAzyme Activity of G4–Hemin Conjugates Obtained via Two Hemin Attachment Methods. Molecules 2018, 23, 1400. https://doi.org/10.3390/molecules23061400
Kosman J, Żukowski K, Juskowiak B. Comparison of Characteristics and DNAzyme Activity of G4–Hemin Conjugates Obtained via Two Hemin Attachment Methods. Molecules. 2018; 23(6):1400. https://doi.org/10.3390/molecules23061400
Chicago/Turabian StyleKosman, Joanna, Krzysztof Żukowski, and Bernard Juskowiak. 2018. "Comparison of Characteristics and DNAzyme Activity of G4–Hemin Conjugates Obtained via Two Hemin Attachment Methods" Molecules 23, no. 6: 1400. https://doi.org/10.3390/molecules23061400