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Cytotoxic, Anti-Proliferative and Apoptosis Activity of l-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells

Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Selangor Darul Ehsan, Malaysia
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Academic Editor: Jean-Marc Sabatier
Molecules 2018, 23(6), 1388; https://doi.org/10.3390/molecules23061388
Received: 20 April 2018 / Revised: 24 May 2018 / Accepted: 31 May 2018 / Published: 8 June 2018
The aim of this study is to investigate the potential anti-cancer activity of l-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time- and dose-dependent cytotoxic activity and the EC50 value was determined at 13 µg/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer. View Full-Text
Keywords: l-amino acid oxidase; Cryptelytrops purpureomaculatus; cytotoxicity; apoptosis; human colon cancer cells l-amino acid oxidase; Cryptelytrops purpureomaculatus; cytotoxicity; apoptosis; human colon cancer cells
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MDPI and ACS Style

Zainal Abidin, S.A.; Rajadurai, P.; Hoque Chowdhury, M.E.; Othman, I.; Naidu, R. Cytotoxic, Anti-Proliferative and Apoptosis Activity of l-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells. Molecules 2018, 23, 1388. https://doi.org/10.3390/molecules23061388

AMA Style

Zainal Abidin SA, Rajadurai P, Hoque Chowdhury ME, Othman I, Naidu R. Cytotoxic, Anti-Proliferative and Apoptosis Activity of l-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells. Molecules. 2018; 23(6):1388. https://doi.org/10.3390/molecules23061388

Chicago/Turabian Style

Zainal Abidin, Syafiq A., Pathmanathan Rajadurai, Md. E. Hoque Chowdhury, Iekhsan Othman, and Rakesh Naidu. 2018. "Cytotoxic, Anti-Proliferative and Apoptosis Activity of l-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells" Molecules 23, no. 6: 1388. https://doi.org/10.3390/molecules23061388

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