Next Article in Journal
Synthesis of the Sex Pheromone of the Tea Tussock Moth Based on a Resource Chemistry Strategy
Next Article in Special Issue
Direct Observation of Hydrangea Blue-Complex Composed of 3-O-Glucosyldelphinidin, Al3+ and 5-O-Acylquinic Acid by ESI-Mass Spectrometry
Previous Article in Journal
Vermicompost Supplementation Improves the Stability of Bioactive Anthocyanin and Phenolic Compounds in Clinacanthus nutans Lindau
Previous Article in Special Issue
(−)-Epigallocatechin 3-Gallate Synthetic Analogues Inhibit Fatty Acid Synthase and Show Anticancer Activity in Triple Negative Breast Cancer
Open AccessFeature PaperArticle

Simultaneous Quantification of Ellagitannins and Related Polyphenols in Geranium thunbergii Using Quantitative NMR

Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan
Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry, Pharmaceutical Sciences, 1-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan
Author to whom correspondence should be addressed.
Molecules 2018, 23(6), 1346;
Received: 18 May 2018 / Revised: 1 June 2018 / Accepted: 2 June 2018 / Published: 4 June 2018
Compared to commonly employed liquid chromatography-based methods, quantitative nuclear magnetic resonance (qNMR) is a recently developed method for accurate quantification of natural compounds in extracts. The simultaneous quantification of ellagitannins and the related polyphenols of Geranium thunbergii were studied using qNMR after a short-term and long-term decoction. The qNMR fingerprint for quantifying ellagitannin was presented in this work. Geraniin was observed in the short-term decoction as a major component while corilagin was the major component of the long-term decoction. An aqueous acetone extract of G. thunbergii after long-term decoction was extracted with diethyl ether, ethyl acetate, and n-butanol. Corilagin was found as a major constituent in the ethyl acetate and n-butanol extracts. Furthermore, the contents of these polyphenols in G. thunbergii from six locations in Japan and three locations in China were quantified. The contents of geraniin and corilagin in G. thunbergii from Japan were higher than those from China. Our finding raised the possibility that qNMR can be effectively employed as a simple, accurate, and efficient method for quantification of ellagitannins in medicinal plants. View Full-Text
Keywords: 1H-NMR; quantitative NMR; ellagitannin; Geranium thunbergii; geraniin 1H-NMR; quantitative NMR; ellagitannin; Geranium thunbergii; geraniin
Show Figures

Graphical abstract

MDPI and ACS Style

Bastian, F.; Ito, Y.; Ogahara, E.; Ganeko, N.; Hatano, T.; Ito, H. Simultaneous Quantification of Ellagitannins and Related Polyphenols in Geranium thunbergii Using Quantitative NMR. Molecules 2018, 23, 1346.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop