Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells
AbstractAleuritolic acid (AA) is a triterpene that is isolated from the root of Croton crassifolius Geisel. In the present study, the cytotoxic effects of AA on hepatocellular carcinoma cells were evaluated. AA exerted dose- and time-dependent cytotoxicity by inducing mitochondria-dependent apoptosis in the hepatocellular carcinoma cell line, HepG2. Meanwhile, treatment with AA also caused dysregulation of autophagy, as evidenced by enhanced conversion of LC3-I to LC3-II, p62 accumulation, and co-localization of GFP and mCherry-tagged LC3 puncta. Notably, blockage of autophagosome formation by ATG5 knockdown or inhibitors of phosphatidylinositol 3-kinase (3-MA or Ly294002), significantly reversed AA-mediated cytotoxicity. These data indicated that AA retarded the clearance of autophagic cargos, resulting in the production of cytotoxic factors and led to apoptosis in hepatocellular carcinoma cells. View Full-Text
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Yi, H.; Wang, K.; Du, B.; He, L.; HO, H.; Qiu, M.; Zou, Y.; Li, Q.; Jin, J.; Zhan, Y.; Zhao, Z.; Liu, X. Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells. Molecules 2018, 23, 1338.
Yi H, Wang K, Du B, He L, HO H, Qiu M, Zou Y, Li Q, Jin J, Zhan Y, Zhao Z, Liu X. Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells. Molecules. 2018; 23(6):1338.Chicago/Turabian Style
Yi, Hua; Wang, Kun; Du, Biaoyan; He, Lina; HO, Hiuting; Qiu, Maosong; Zou, Yidan; Li, Qiao; Jin, Junfeng; Zhan, Yujuan; Zhao, Zhongxiang; Liu, Xiaodong. 2018. "Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells." Molecules 23, no. 6: 1338.
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