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Article

Sulforaphane Modulates Joint Inflammation in a Murine Model of Complete Freund’s Adjuvant-Induced Mono-Arthritis

1
Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil
2
Florence Institute, Imunology Departament São Luis 65075, Brazil
3
Pathology Departament, State University of Maranhao, Sao Luis 65075, MA, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2018, 23(5), 988; https://doi.org/10.3390/molecules23050988
Received: 11 January 2018 / Revised: 20 March 2018 / Accepted: 23 March 2018 / Published: 24 April 2018
Rheumatoid arthritis (RA) is characterized by inflammation of one or more joints, and affects ~1% of the adult population worldwide. Sulforaphane (SFN) is a natural compound that has been suggested as an antioxidant. Here, SFN’s effects were evaluated in a murine mono-arthritis model. Mono-arthritis was induced in mice by a single intra-articular injection of Complete Freund’s Adjuvant (CFA-10 µg/joint, in 10 µL) into the ipsilateral joint. The contralateral joint received an equal volume of PBS. On the 4th day post-joint inflammation induction, animals received either SFN (10 mg/kg) or vehicle (3% DMSO in saline), intraperitoneally (i.p.), twice a day for 3 days. Joint swelling and secondary mechanical allodynia and hyperalgesia were evaluated over 7 days post-CFA. After this period, animals were culled and their blood and synovial fluid samples were collected for analysis of cell populations, cytokine release and thioredoxin reductase (TrxR) activity. Knee joint samples were also collected for histology. SFN reduced joint swelling and damage whilst increasing the recruitment of Ly6C+ and Ly6G+ cells to CFA-injected joints. SFN-treated animals presented down-regulation of CD11b and CD62L on synovial fluid Ly6G+ cells. Synovial fluid samples obtained from CFA-injected joints and plasma samples of SFN-treated mice presented higher levels of IL-6 and increased activity of TrxR, in comparison with controls. These results indicate that SFN reduces knee joint damage by modulating cell activation/migration to the joints, cytokine production and increasing the activity of TrxR, and therefore, may represent an alternative treatment to joint inflammation. View Full-Text
Keywords: rheumatoid arthritis; sulforaphane; oedema; IL-6; thioredoxin reductase rheumatoid arthritis; sulforaphane; oedema; IL-6; thioredoxin reductase
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MDPI and ACS Style

Silva Rodrigues, J.F.; Santos Silva e Silva Figueiredo, C.; França Muniz, T.; Silva de Aquino, A.F.; Neuza da Silva Nina, L.; Fialho Sousa, N.C.; Nascimento da Silva, L.C.; De Souza, B.G.G.F.; Da Penha-Silva, T.A.; Abreu-Silva, A.L.; De Sá, J.C.; Soares Fernandes, E.; Grisotto, M.A.G. Sulforaphane Modulates Joint Inflammation in a Murine Model of Complete Freund’s Adjuvant-Induced Mono-Arthritis. Molecules 2018, 23, 988. https://doi.org/10.3390/molecules23050988

AMA Style

Silva Rodrigues JF, Santos Silva e Silva Figueiredo C, França Muniz T, Silva de Aquino AF, Neuza da Silva Nina L, Fialho Sousa NC, Nascimento da Silva LC, De Souza BGGF, Da Penha-Silva TA, Abreu-Silva AL, De Sá JC, Soares Fernandes E, Grisotto MAG. Sulforaphane Modulates Joint Inflammation in a Murine Model of Complete Freund’s Adjuvant-Induced Mono-Arthritis. Molecules. 2018; 23(5):988. https://doi.org/10.3390/molecules23050988

Chicago/Turabian Style

Silva Rodrigues, João F., Cristiane Santos Silva e Silva Figueiredo, Thayanne França Muniz, Alana F. Silva de Aquino, Larissa Neuza da Silva Nina, Nagila C. Fialho Sousa, Luis C. Nascimento da Silva, Breno G.G.F. De Souza, Tatiane A. Da Penha-Silva, Ana L. Abreu-Silva, Joicy C. De Sá, Elizabeth Soares Fernandes, and Marcos A.G. Grisotto 2018. "Sulforaphane Modulates Joint Inflammation in a Murine Model of Complete Freund’s Adjuvant-Induced Mono-Arthritis" Molecules 23, no. 5: 988. https://doi.org/10.3390/molecules23050988

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