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Molecules 2018, 23(5), 1220; https://doi.org/10.3390/molecules23051220

Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity against Human Cells of a Peptide Derived from Bovine αS1-Casein

1
Key Laboratory of Dairy Science, Northeast Agricultural University, College of Food Science, Harbin 150030, China
2
Harbin Veterinary Research Institute, CAAS, Harbin 150001, China
*
Author to whom correspondence should be addressed.
Received: 18 April 2018 / Revised: 5 May 2018 / Accepted: 17 May 2018 / Published: 19 May 2018
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Abstract

The primary objective of this study was to improve our understanding of the antimicrobial mechanism of protein-derived peptides and to provide evidence for protein-derived peptides as food bio-preservatives by examining the antimicrobial activities, low cytotoxicity, stabilities, and mechanism of Cp1 (LRLKKYKVPQL). In this study, the protein-derived peptide Cp1 was synthesized from bovine αS1-casein, and its potential use as a food biopreservative was indicated by the higher cell selectivity shown by 11-residue peptide towards bacterial cells than human RBCs. It also showed broad-spectrum antimicrobial activity, with minimum inhibitory concentrations (MICs) of 64–640 μM against both gram-positive and gram-negative bacteria. The peptide had low hemolytic activity (23.54%, 512 μM) as well as cytotoxicity. The results of fluorescence spectroscopy, flow cytometry, and electron microscopy experiments indicated that Cp1 exerted its activity by permeabilizing the microbial membrane and destroying cell membrane integrity. We found that Cp1 had broad-spectrum antimicrobial activity, low hemolytic activity, and cytotoxicity. The results also revealed that Cp1 could cause cell death by permeabilizing the cell membrane and disrupting membrane integrity. Overall, the findings presented in this study improve our understanding of the antimicrobial potency of Cp1 and provided evidence of the antimicrobial mechanisms of Cp1. The peptide Cp1 could have potential applications as a food biopreservative. View Full-Text
Keywords: antibacterial peptides; antimicrobial activity; hemolytic activity; human cell; cytotoxicity mechanism antibacterial peptides; antimicrobial activity; hemolytic activity; human cell; cytotoxicity mechanism
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Hou, J.; Liu, Z.; Cao, S.; Wang, H.; Jiang, C.; Hussain, M.A.; Pang, S. Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity against Human Cells of a Peptide Derived from Bovine αS1-Casein. Molecules 2018, 23, 1220.

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