Mitochondria Transcription Factor A: A Putative Target for the Effect of Melatonin on U87MG Malignant Glioma Cell Line
Abstract
:1. Introduction
2. Results
2.1. Melatonin Decreased the Expression of TFAM, TFB1M, and TFB2M
2.2. Melatonin Decreased the Content of TFAM Protein
2.3. Melatonin Decreased the Transcription of mtDNA but Did Not Affect Replication
2.4. Melatonin Induced ROS Production
2.5. Melatonin Induced Mitochondria Depolarization and Apoptosis
2.6. Melatonin Arrested U87MG Cells at the G0/G1 Phase of the Cell Cycle
2.7. Melatonin Potentiated the Effect of Temozolomide (TMZ) to Reduce Cell Viability
3. Discussion
4. Materials and Methods
4.1. Cell Culture Conditions
4.2. Extraction of RNA and DNA
4.3. Expression of TFAM, TFB1M, TFB2M, and NADH Dehydrogenase 1 (MT-ND1) by qRT-PCR
4.4. Mitochondrial DNA Copy Number Quantification
4.5. Western Blot Analysis
4.6. Evaluation of Oxidative Stress, Cell Cycle, Apoptosis, and Mitochondria Polarization
4.7. Cell Viability/Proliferation
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Franco, D.G.; Moretti, I.F.; Marie, S.K.N. Mitochondria Transcription Factor A: A Putative Target for the Effect of Melatonin on U87MG Malignant Glioma Cell Line. Molecules 2018, 23, 1129. https://doi.org/10.3390/molecules23051129
Franco DG, Moretti IF, Marie SKN. Mitochondria Transcription Factor A: A Putative Target for the Effect of Melatonin on U87MG Malignant Glioma Cell Line. Molecules. 2018; 23(5):1129. https://doi.org/10.3390/molecules23051129
Chicago/Turabian StyleFranco, Daiane G., Isabele F. Moretti, and Suely K. N. Marie. 2018. "Mitochondria Transcription Factor A: A Putative Target for the Effect of Melatonin on U87MG Malignant Glioma Cell Line" Molecules 23, no. 5: 1129. https://doi.org/10.3390/molecules23051129