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Molecules 2018, 23(5), 1078; doi:10.3390/molecules23051078
Three New Highly Oxygenated Germacranolides from Carpesium Divaricatum and Their Cytotoxic Activity
Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China
School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China
Author to whom correspondence should be addressed.
Received: 30 March 2018 / Accepted: 27 April 2018 / Published: 3 May 2018
Three new highly oxygenated (2–4), and two known (1 and 5) germacranolides, were isolated from the whole plant of Carpesium divaricatum. The planar structures and relative configurations of the new compounds were determined by detailed spectroscopic analysis. The absolute configuration of 1 was established using the circular dichroism (CD) method and X-ray diffraction, and the stereochemistry of the new compounds 2–4 were determined using similar CD spectra with 1. The new compound 2 and the known compound 5 exhibited potent cytotoxicity against hepatocellular cancer (Hep G2) and human cervical cancer (HeLa) cells, superior to those of the positive control cis-platin.
Keywords:Carpesium divaricatum; germacranolides; absolute configuration; cytotoxicity
The genus Carpesium (Asteraceae) includes 25 species worldwide, most of which are distributed across Asia and Europe, particularly in southwest China [1,2]. In China, Korea, and Japan, many Carpesium species have been used for the treatment of fevers, colds, bruises, and snake bites, due to their antipyretic, analgesic, vermifugic, hemostatic, detoxifying, and anti-inflammatory properties . The genus is rich in diverse sesquiterpenoid lactones, such as eudesmanolides and germacranolides [2,3,4,5,6]. Previous investigations indicate that sesquiterpenoid lactones possessing an α-methylene-γ-lactone moiety are cytotoxic to human cancer cells [2,3,4,5,6,7]. Recently, six sesquiterpenoid lactones with new skeletons displaying significantly cytotoxic activity were isolated from Carpesium plants [8,9].
Carpesium divaricatum Sieb.et Zucc is widely distributed in China, and is traditionally used for the treatment of fevers, colds, bruises, insect bites and inflammatory diseases [10,11,12,13,14,15]. Previous investigations of this plant reported the isolation of germacrane-type sesquiterpene lactones [4,14,15,16,17]. The parent nucleus of the germacranes contains a 10-membered ring with different post-modifications to produce diverse structural features. Many skeletal types of germacranolides with broad biological activities, such as cytotoxic and anti-inflammatory properties, have been isolated from the genera Carpesium, Inula and Allagopappus [5,6,11,15,16,17,18,19,20,21,22,23,24,25,26]. Our previous study led to the isolation, structural elucidation and analysis of the cytotoxic activity of eight germacranolides from this plant .
As a part of our ongoing search for new bioactive products from medicinal plants in China, three new, and two known highly oxygenated germacranolides representing other subtype (Figure 1), were isolated from the whole plant of C. divaricatum. In this paper, a structural elucidation and bioactive evaluation of these compounds is presented.
2. Results and Discussion
2.1. Purification of Compounds 1–5
The whole plant of C. divaricatum was extracted three times with EtOH–H2O (95:5). The five highly oxygenated germacranolides were isolated and purified via silica gel chromatograpy, Sephadex LH-20 gel chromatograpy, and semi-preparative High-Performance Liquid Chromatography (HPLC).
2.2. Structure Elucidation of Compounds 1–5
Compound 1 was identified as 4β,8α-dihydroxy-5β-isobutyryloxy-9β-3-methylbutyryloxy-3-oxo- germacran-6α, 12-olide (1), by comparison of its MS, NMR data, as well as optical rotation data, with reported data (supplementary materials Figure S1) . However, its absolute configuration has not been determined. According to Beecham’s rule, the CD spectrum (Figure 2) of 1 exhibited a positive Cotton effect near 254 nm (α-methylene-γ-lactone region), supporting 6S, 7R configuration . Fortunately, a suitable crystal was obtained for X-ray diffraction to confirm the absolute configuration. The X-ray crystallographic analysis [flack parameter: 0.08(17)] established unambiguously the absolute configuration of 1 to be 4R, 5R, 6S, 7R, 8R, 9R and 10R (Figure 3). Herein, the absolute configuration of 1 is reported for the first time.
Compound 2 was obtained as white needles. The molecular formula was assigned as C25H38O9, on the basis of the positive-ion HRESIMS peak at m/z 505.2427 [M + Na]+, together with its 1H and 13C NMR data (Table 1). Its IR spectrum showed hydroxy (3458 cm-1) and carbonyl (1744 and 1718 cm−1) absorptions. The 1H and 13C NMR spectra of 2 showed an α-methylene-γ-lactone at δH 6.27 (1H, d, J = 3.0 Hz, Ha-13) and 5.62 (1H, d, J = 3.0 Hz, Hb-13), δC 132.6 (C-11), 123.9 (C-13) and 169.7 (C-12); three carbonyl carbons at δC 217.8 (C-3), 172.5 (C-1′) and 173.3 (C-1′′); one oxygenated tertiary carbon at 80.3 (C-4); four oxygenated methines at δH 5.36 (1H, dd, J = 8.5, 2.0 Hz, H-5), 4.60 (1H, dd, J = 8.5, 5.0 Hz, H-6), 4.35 (1H, d, J = 10.5 Hz, H-8), and 5.11 (1H, d, J = 10.5 Hz, H-9), δC 78.2 (C-5), 79.8 (C-6), 70.3 (C-8), and 78.7 (C-9); and two methyl groups at δH 0.92 (3H, d, J = 7.0 Hz, CH3-14), 1.18 (3H, s, CH3-15). These signals (1H and 13C NMR data) implied that the structure of 2 was similar to that of 1, except that the isobutyryloxy group of 1 was replaced by a 3-methylbutyryloxy group at C-5 in 2; this was further confirmed by the 1H-1H COSY, HSQC, and HMBC spectra (Figure 4). On the basis of these data, the planar structure of 2 was established.
The relative configuration of 2 was determined by analysis of ROESY data. The key NOE correlations of H-8/H-6, H-7/H-10, H-7/H-5, H-7/H-9, and H-5/H3-15 indicated that 2 had the same relative configuration as 1 (Figure 4). The CD spectrum of 2 showed positive Cotton effects at near 254 nm, which closely resembled those of 1. Similar ROESY and CD data of 2 and 1 (Figure 2) assigned the absolute configuration of 2 as 4R, 5R, 6S, 7R, 8R, 9R, and 10R. Thus, the structure of compound 2, named divarolide E, is defined as shown.
Compounds 3–4 possessed molecular formulas of C23H32O9 and C25H38O9, from their HRESIMS at m/z 475.1939 [M + Na]+ and m/z 505.2414 [M + Na]+ respectively. The 1H and 13C NMR data of 3–4 were similar to those of 1, except that the 2-methacryloyloxy group at C-9 in 3 was observed in place of 3-methylbutyryloxy group in 1, and an isobutyryloxy group at C-5 and the 3-methylbutyryloxy group at C-9 in 1 were replaced by two 2-methylbutyryloxy groups in 4, respectively. These observations were confirmed by analyses of relevant 1H-1H COSY, HSQC and HMBC data (Table 1). The relative configurations of 3–4 were determined to be the same as those of 1, by comparison of ROESY data for relevant protons. Similar CD data of 3–4 and 1 (Figure 2) revealed the same absolute configurations of 3–4 as that of 1. Thus, the structures of compounds 3–4 were established as shown, and named divarolide F and divarolide G respectively.
The structure of the known compound (5) was identified as 4β,8α-dihydroxy-5β-2-methylbutyryloxy-9β-3-methylbutyryloxy-3-oxo-germacran-7β, 12α-olide , by comparison of its spectroscopic data with reported data.
2.3. In Vitro Cytotoxic Activities of Compounds 1–5
Compounds 1–5 were evaluated for their cytotoxic activity against human cervical cancer (HeLa), hepatocellular cancer (Hep G2), and lung cancer (A549) cell lines (Table 2). The new compound 2, and the known compound 5, exhibited cytotoxicity against Hep G2 (IC50 values of 7.47 μM) and HeLa (IC50 values of 16.82 μM) cell lines, and the IC50 values were lower than those of the positive control cis-platin (IC50 values of 13.03, and 15.34 μM respectively). In addition, 1 and 2 also displayed strong cytotoxicity against Hep G2, with an IC50 value of 16.98 μM, and HeLa with an IC50 value of 16.82 μM.
3. Materials and Methods
3.1. General Experimental Procedures
Optical rotations were measured on a Perkin-Elmer 241 polarimeter (Perkin-Elmer, Waltham, MA, USA), and UV spectra were recorded on Shimadzu UV-2501 PC (Shimadzu, Kyoto, Japan). IR data were recorded using a Shimadzu FTIR-8400S spectrophotometer (Shimadzu, Kyoto, Japan). 1H and 13C NMR data were acquired using Bruker 500 and Bruker 600 instruments (Bruker, Rheinstetten, Germany), with solvent signals (CD3OD: δH 3.30/δC 49.0 ppm;) as references. HRESIMS data were acquired using a Q-TOF analyzer in SYNAPT HDMS system (Waters, Milford, MA, USA). X-ray diffraction data were collected on the Agilent GEMINITME instrument (CrysAlisPro software, Version 220.127.116.11; Agilent, Santa Clara, CA, USA). HPLC was performed using Waters 2535 system (Waters, Milford, MA, USA), with the following components: preparative column, a Daisogel-C18-100A (10 μm, 30 × 250 mm, ChuangXinTongHeng Sci. & Tech., Beijing, China) and a YMC-Pack ODS-A column (5 μm, 10 × 250 mm, YMC, Kyoto, Japan); and detector, Waters 2489 UV. Sephadex LH-20 (40–70 μm, Pharmacia Biotech AB, Uppsala, Sweden), silica gel (60–100, 100–200 and 200–300 mesh) and silica gel GF254 sheets (0.20–0.25 mm) (Qingdao Marine Chemical Plant, Qingdao, China) were used for column chromatography and TLC, respectively. TLC spots were visualized under UV light and by dipping into 5% H2SO4 in EtOH, followed by heating.
3.2. Plant Material
The whole plants of C. divaricatum were collected from EnShi, Hubei province (China) in August of 2013. They were identified by Prof. Ben-Gang Zhang of Institute of Medicinal Plant Development. A voucher specimen (No. 20130828) was deposited in the National Compound Library of Traditional Chinese Medicines, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Beijing, China.
3.3. Isolation and Purification of Compounds 1–5
The air-dried plants (9 kg) were extracted three times (7 days each time) with EtOH–H2O (95:5) at room temperature. The combined extract was concentrated under reduced pressure to furnish a dark brown residue (570 g), which was suspended in H2O and partitioned in turn with petroleum ether (bp 60–90 °C), EtOAc, and n-BuOH. The EtOAc extract (207 g) was separated chromatographically on silica gel column (60–100 mesh, 16 × 20 cm) with a gradient mixture of CH2Cl2–MeOH (100:1, 60:1, 30:1, 15:1 and 6:1) as eluent. Five fractions were collected according to TLC analysis. Fraction A (CH2Cl2–MeOH, 100:1, 140 g) was separated by silica gel column chromatography (CC) (100–200 mesh, 16 × 20 cm) with petroleum ether–aceton (50:1, 25:1, 20:1, 15:1, 12:1, 10:1, 7:1, 5:1, 3:1 and 1:1) as eluent to give fractions A1–A11. Fraction A10 (petroleum ether–aceton, 3:1, 40 g) was separated by Sephadex LH-20 CC (5 × 200 cm, MeOH) to give Fr.A10S1–Fr.A10S3. Fraction A10S2 (20 g) was then subjected to MCI gel CC (6 × 50 cm) with a gradient mixture of MeOH–H2O (60:40, 80:20, and 100:0, 4000 mL each) to give three fractions (Fr.A10S2M1–Fr.A10S2M3).
Fraction A10S2M2 (13 g) was further separated chromatographically on silica gel column (200–300 mesh, 5 × 50 cm) with a gradient mixture of CH2Cl2–MeOH (150:1, 100:1, 50:1 and 20:1) as eluent, and a total of 86 fractions (Fr.A10S2M2-1–86, 200 mL each) were collected. Fraction A10S2M2-34–50 (1.5 g) were separated by preparative HPLC (20 mL/min, 70% MeOH in H2O) and semipreparative HPLC (2 mL/min, 52–75% MeOH in H2O for 25 min and followed by 75–95% MeOH in H2O for 10 min; 2 mL/min, 40–80% MeCN in H2O for 40 min) to yield 3 (5 mg). Fraction A10S2M2-74–79 (140 mg) were purified using semipreparative HPLC (2 mL/min, 60–80% MeOH in H2O for 25 min and followed by 80–90% MeOH in H2O for 20 min; 2 mL/min, 30–70% MeCN in H2O for 40 min) and to yield 1 (30 mg).
Fraction A9 (petroleum ether-Aceton, 5:1, 30 g) was separated by Sephadex LH-20 CC (5 × 200 cm, MeOH) to give Fr.A9S1–Fr.A9S3. Fraction A9S2 (20 g) was then subjected to MCI gel CC (6 × 50 cm) with a gradient mixture of MeOH–H2O (60:40, 80:20, and 100:0, 4000 mL each) to give three fractions (Fr.A9S2M1–Fr.A9S2M3). Fraction A9S2M2 (10 g) was further separated chromatographically on a silica gel column (100–200 mesh, 5 × 50 cm), with a gradient mixture of petroleum ether–Aceton (10:1, 7:1, 5:1, 3.5:1, 2:1 and 1:1) as eluent; a total of 200 fractions (Fr.A9S2M2-1–200, 50 mL each) were collected. Fraction A9S2M2-107–112 (2.5 g) were separated by silica gel column chromatography (CC) (200–300 mesh, 5 × 40 cm) with CH2Cl2–MeOH (150:1, 75:1, 30:1, and 15:1) as eluent to give Fr. A9S2M2-107–112-A1–Fr. A9S2M2-107–112-A8. Fraction A9S2M2-107–112–A3 (CH2Cl2–MeOH, 75:1, 500 mg) was further purified using semipreparative HPLC (2 mL/min, 65–90% MeOH in H2O for 40 min; 2 mL/min, 40–80% MeCN in H2O for 40 min) to yield 2 (4.5 mg), 4 (5 mg) and 5 (10 mg).
3.4. Characterization of Compounds 2–4
Divarolide E (2): white needles (CH3OH), [α] –95.2 (c 0.125, MeOH); UV (MeOH) λmax (logε): 210 (3.38) nm, IR (KBr) νmax: 3458, 1744, 1718, 1661 cm−1; CD (MeOH) 215 (Δε −0.083), 308 (Δε −0.013) nm; HRESIMS (pos.): m/z 505.2427 [M + Na]+ (calcd for C25H38O9Na, 505.2414); 1H NMR and 13C NMR data, see Table 1.
Divarolide F (3): white needles (CH3OH), [α] –78.7 (c 0.150, MeOH); UV (MeOH) λmax (logε): 200 (4.68) nm, IR (neat) νmax: 3463, 1762, 1707, 1647 cm−1; CD (MeOH) 215 (Δε −0.122), 307 (Δε −0.021) nm; HRESIMS (pos.): m/z 475.1939 [M + Na]+ (calcd for C23H32O9Na, 475.1944); 1H NMR and 13C NMR data, see Table 1.
Divarolide G (4): white needles (CH3OH), [α] –84.7 (c 0.085, MeOH); UV (MeOH) λmax (logε): 209 (4.00) nm, IR (neat) νmax: 3440, 2969, 1740, 1660 cm−1; CD (MeOH) 215 (Δε −0.099), 307 (Δε −0.016) nm; HRESIMS (pos.): m/z 505.2414 [M + Na]+ (calcd for C25H38O9Na, 505.2414); 1H NMR and 13C NMR data, see Table 1.
3.5. X-ray Crystal Structure Analysis of Compound 1
X-ray diffraction data were collected on the Agilent GEMINITME instrument (CrysAlisPro software, Version 18.104.22.168), with enhanced Cu Kα radiation (λ = 1.54184 Å). The structure was solved by direct methods and refined by full-matrix least-squares techniques (SHELXL-97). All non-hydrogen atoms were refined with anisotropic thermal parameters. Hydrogen atoms were located by geometrical calculations and from positions in the electron density maps. Crystallographic data (excluding structure factors) for 1 in this paper has been deposited with the Cambridge Crystallographic Data Centre (deposition number CCDC 1570798). Copies of the data can be obtained, free of charge, on application to CCDC, 12 Union Road, Cambridge CB2 1EZ, UK (fax: +44-12-23336033 or e-mail: firstname.lastname@example.org).
A colorless monoclinic crystal (0.22 × 0.18 × 0.03 mm) of 1 was grown from MeOH-H2O (3:1). Crystal data: C24H36O9, M = 471.55, T = 106.8 K, triclinic, space groupP21, a = 14.2950(7) Å, b = 9.5219(4) Å, c = 18.7748(11) Å, α = 90.00°, β = 104.713°, γ = 90.00°, V = 2471.7(2) Å3, Z = 4, ρ = 1.267 mg/mm3, μ(Cu Kα) = 0.805 mm−1, measured reflections = 18092, unique reflections = 9353 (Rint = 0.0470), largest difference peak/hole = 0.363/−0.247 e Å−3, and flack parameter = 0.08(17). The final Rindexes [I > 2σ (I)] were R1 = 0.0535, and wR2 = 0.1288. The final Rindexes (all data) were R1 = 0.0658, and wR2 = 0.1390. The goodness of fit on F2 was 1.007.
3.6. Cytotoxicity Assays of Compounds 1–5
Cell cultures: Human A549, HepG2, and HeLa cell lines from Cancer Institute and Hospital of Chinese Academy of Medical Sciences (Beijing, China), were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, CA, USA) supplemented with 10% (v/v) fetal calf serum (Gibco, CA, USA), penicillin G (Macgene, China) 100 units mL−1 and streptomycin (Macgene, China) 100 μg mL−1, at 37 °C under 5% CO2.
Cell viability assay: The assay was run in triplicate. In a 96-well plate, each well was plated with 2 × 104 cells. After cell attachment overnight, the medium was removed, and each well was treated with 100 μL of medium containing 0.1% DMSO or different concentrations of the test compounds and the positive control cis-platin. The plate was incubated at 37 °C for 4 days in a humidified, 5% CO2 atmosphere. Cytotoxicity was determined using a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay . After the addition of a 10 μL MTT solution (5 mg/mL), cells were incubated at 37 °C for 4 h. After adding 150 μL DMSO, cells were shaken to mix thoroughly. The absorbance of each well was measured at 540 nm in a Multiscan photometer. The IC50 values were calculated by Origin software and listed in Table 2.
Statistical analysis: Values were expressed as mean ± SD. Statistical analyses were performed using the Student’s t-test. Differences were considered significant when associated with a probability of 5% or less (p ≤ 0.05).
In conclusion, three new compounds (2–4), as well as two known compounds (1 and 5), were isolated from the whole plant of C. divaricatum. Structurally, all compounds contained a 5-membered γ-lactone ring fused to a circular 10-membered carbocycle. We obtained a set of isomers (2/4/5) from the same plant. The isolation of these isomers is a huge challenge because they are highly oxygenated and have similar structures. The absolute configuration of compound 1 was unambiguously established by X-ray diffraction. The other compounds with the same skeleton were determined by comparison of NOESY and CD data with those of 1. Compounds 1 and 5 showed significant cytotoxicity against two human tumor cell lines. These findings are an important addition to the present knowledge on the structurally diverse and biologically significant germacranolide family.
The Supplementary Materials are available online.
T.Z. and Z.-M.Z. conceived and designed the experiments; T.Z., Q.-B.Z., J.-G.S., J.-H.C., and G.D. performed the experiments; H.-W.Z. and H.-M.J. analyzed the data; T.Z. and Z.-M.Z. wrote the paper.
This work was financially supported by the CAMS Innovation Fund for Medical Sciences (CIFMS, 2016-I2M-1-010 and 2016-I2M-2-003), the Chinese National S&T Special Project on Major New Drug Innovation (2017ZX09301059), and the National Key Research and Development Program of China (2017YFD0201400-2).
Conflicts of Interest
There is no conflict of interest associated with the authors of this paper.
- Mabberley, D.J. Mabberley′s Plant Book, 3rd ed.; Cambridge University Press: Cambridge, UK, 2008; p. 154. [Google Scholar]
- Zhang, J.P.; Wang, G.W.; Tian, X.H.; Yang, Y.X.; Liu, Q.X.; Chen, L.P.; Li, H.L.; Zhang, W.D. The genus Carpesium: A review of its ethnopharmacology, phytochemistry and pharmacology. J. Ethnopharmacol. 2015, 163, 173–191. [Google Scholar] [CrossRef] [PubMed]
- Yang, C.; Yuan, C.S.; Jia, Z.J. Xanthanolides, germacranolides, and other constituents from Carpesium longifolium. J. Nat. Prod. 2003, 66, 1554–1557. [Google Scholar] [CrossRef] [PubMed]
- Xie, W.D.; Wang, X.R.; Ma, L.S.; Li, X.; Row, K.H. Sesquiterpenoids from Carpesium divaricatum and their cytotoxic activity. Fitoterapia 2012, 83, 1351–1355. [Google Scholar] [CrossRef] [PubMed]
- Gao, X.; Lin, C.J.; Jia, Z.J. Cytotoxic germacranolides and acyclic diterpenoides from the seeds of Carpesium triste. J. Nat. Prod. 2007, 70, 830–834. [Google Scholar] [CrossRef] [PubMed]
- Lin, Y.L.; Ou, J.C. Napalolides A–D, four new sesquiterpene lactones from Carpesium nepalense. J. Nat. Prod. 1996, 59, 991–993. [Google Scholar] [CrossRef]
- Li, X.W.; Weng, L.; Gao, X.; Zhao, Y.; Pang, F.; Liu, J.H.; Zhang, H.F.; Hu, J.F. Antiproliferative and apoptotic sesquiterpene lactones from Carpesium faberi. Bioorg. Med. Chem. Lett. 2011, 21, 366–372. [Google Scholar] [CrossRef] [PubMed]
- Yang, Y.X.; Shan, L.; Liu, Q.X.; Shen, Y.H.; Zhang, J.P.; Ye, J.; Xu, X.K.; Li, H.J.; Zhang, W.D. Carpedilactones A-D, four new isomeric sesquiterpene lactone dimers with potent cytotoxicity from Carpesium faberi. Org. Lett. 2014, 16, 4216–4219. [Google Scholar] [CrossRef] [PubMed]
- Wu, J.W.; Tang, C.P.; Chen, L.; Qiao, Y.; Geng, M.Y.; Ye, Y. Dicarabrones A and B, a pair of new epimers dimerized from sesquiterpene lactones via a [3 + 2] cycloaddition from Carpesium abrotanoides. Org. Lett. 2015, 17, 1656–1659. [Google Scholar] [CrossRef] [PubMed]
- Editorial Committee of the Administration Bureau of Traditional Chinese Medicine. Chinese Materia Medica; Shanghai Science &Technology Press: Shanghai, China, 1999; Volume 21, p. 761. [Google Scholar]
- Kim, E.J.; Jin, H.K.; Kim, Y.K.; Lee, H.Y.; Lee, S.Y.; Lee, K.R.; Zee, O.P.; Han, J.W.; Lee, H.W. Suppression by a sesquiterpene lactone from Carpesium divaricatum of inducible nitric oxide synthase by inhibiting nuclear factor-κB activation. Biochem. Pharmacol. 2001, 61, 903–910. [Google Scholar] [CrossRef]
- Zee, O.P.; Kim, D.K.; Choi, S.U.; Lee, C.O.; Lee, K.R. A new cytotoxic acyclic diterpene from Carpesium divaricatum. Arch. Pharm. Res. 1999, 22, 225–227. [Google Scholar] [CrossRef] [PubMed]
- Zee, O.P.; Kim, D.K.; Lee, K.R. Thymol derivatives from Carpesium divaricatum. Arch. Pharm. Res. 1998, 21, 618–620. [Google Scholar] [CrossRef] [PubMed]
- Chung, I.M.; Seo, S.H.; Kang, E.Y.; Park, W.H.; Park, S.D.; Moon, H.I. Antiplasmodial activity of isolated compounds from Carpesium divaricatum. Phytother. Res. 2010, 24, 451–453. [Google Scholar] [CrossRef] [PubMed]
- Kim, D.K.; Beak, N.I.; Choi, S.U.; Lee, C.O.; Lee, K.R.; Zee, O.P. Four new cytotoxic germacranolides from Carpesium divaricatum. J. Nat. Prod. 1997, 60, 1199–1202. [Google Scholar] [CrossRef] [PubMed]
- Maruyama, M. Sesquiterpene lactones from Carpesium divaricatum. Phytochemistry 1990, 29, 547–550. [Google Scholar] [CrossRef]
- Kim, D.K.; Lee, K.R.; Zee, O.P. Sesquiterpene lactones from Carpesium divaricatum. Phytochemistry 1997, 46, 1245–1247. [Google Scholar] [CrossRef]
- Lee, H.J.; Jung, H.; Kwon, J.; Li, H.; Lee, D.Y.; Lim, H.J.; Kim, M.R.; Moon, D.C.; Ryu, J.H. A germacranolide sesquiterpene lactone suppressed inducible nitric oxide synthase by downregulating NF-κB activity. Can. J. Physiol. Pharmacol. 2011, 89, 232–237. [Google Scholar] [CrossRef] [PubMed]
- Moon, H.I.; Zee, O. Antiproliferative effect from sesquiterpene lactones of Carpesium rosulatum MlQ consumed in South Korea on the five human cancer cell lines. Rec. Nat. Prod. 2010, 4, 3149–3155. [Google Scholar]
- Moon, H.I.; Zee, O. Sesquiterpene lactones from Carpesium rosulatum with potential cytotoxicity against five human cancer cell lines. Hum. Exp. Toxicol. 2010, 30, 1083–1087. [Google Scholar] [CrossRef] [PubMed]
- Baruah, R.N.; Sharma, R.P. Thyagarajan, G. Unusual germacranolides from Inula eupatorioides. J. Org. Chem. 1980, 45, 4838–4843. [Google Scholar] [CrossRef]
- Baruah, N.C.; Baruah, R.N.; Sharma, R.P.; Baruah, J.N. Germacranolides of Inula eupatorioides. 2. Absolute configuration of the ineupatorolides. J. Org. Chem. 1982, 47, 137–140. [Google Scholar] [CrossRef]
- Goswami, A.C.; Baruah, R.N.; Sharma, R.P.; Baruah, J.N.; Kulanthaivel, P.; Herz, W. Germacranolides from Inula cappa. Phytochemistry 1984, 23, 367–372. [Google Scholar] [CrossRef]
- Wang, F.Y.; Li, X.Q.; Sun, Q.; Yao, S.; Ke, C.Q.; Tang, C.P.; Liu, H.C.; Geng, M.Y.; Ye, Y. Sesquiterpene lactones from Carpesium divaricatum. Phytochem. Lett. 2012, 5, 639–642. [Google Scholar] [CrossRef]
- Gonzalez, A.G.; Barrera, J.B.; Mendez, J.T.; Martinez, J.E.; Sanchez, M.L. Germacranolides from Allagopappus viscosissimus. Phytochemistry 1992, 31, 330–331. [Google Scholar] [CrossRef]
- Gonzalez, A.G.; Bermejo, J. Sesquiterpene lactones and other constituents of Allagopappus species. J. Nat. Prod. 1995, 58, 432–437. [Google Scholar] [CrossRef]
- Zhang, T.; Si, J.G.; Zhang, Q.B.; Ding, G.; Zou, Z.M. New highly oxygenated germacranolides from Carpesium divaricatum and their cytotoxic activity. Sci. Rep. 2016, 6, 27237. [Google Scholar] [CrossRef] [PubMed]
- Wang, B.J.; Won, S.J.; Su, Z.R.; Yu, C.L. Free radical scavenging and apoptotic effects of cordyceps sinensis fractionated by supercritical carbon dioxide. Food Chem. Toxicol. 2005, 43, 543–552. [Google Scholar] [CrossRef] [PubMed]
- Sample Availability: Samples of the compounds 1 and 5 are available from the authors.
Figure 1. Chemical structures of compounds 1–5.
Figure 2. CD spectra of compounds 1–4.
Figure 3. X-ray ORTEP drawing of 1.
Figure 4. Key 2D correlations of compound 2.
Table 1. NMR spectral data of 2–4.
|No.||2 a||3 b||4 a|
|δC, Type||δH (J in Hz)||δc, Type||δH (J in Hz)||δc, Type||δH (J in Hz)|
|1||25.3 CH2||1.79 m, 1.65 m||25.3 CH2||1.83 m, 1.71 m||25.3 CH2||1.87 m, 1.75 m|
|2||32.8 CH2||3.76 br d (7.5), 2.22 m||32.9 CH2||3.81 br d (7.5), 2.16 m||33.2 CH2||3.87 m, 2.29 m|
|3||217.8 C||217.6 C||217.6 C|
|4||80.3 C||80.4 C||80.3 C|
|5||78.2 CH||5.36 dd (8.5, 2.0)||78.1 CH||5.37 dd (9.6, 1.2)||78.1 CH||5.39 br d (9.5)|
|6||79.8 CH||4.60 dd (8.5, 5.0)||79.9 CH||4.65 dd (9.6, 6.0)||79.9 CH||4.69 dd (9.5,6.5)|
|7||41.5 CH||2.97 m||41.6 CH||3.01 m||41.7 CH||3.02 m|
|8||70.3 CH||4.35 d (10.5)||70.3 CH||4.43 d (10.2)||70.5 CH||4.40 d (10.0)|
|9||78.7 CH||5.11 d (10.5)||79.3 CH||5.18 d (10.2)||78.4 CH||5.15 d (10.0)|
|10||29.8 CH||2.15 m||30.1 CH||2.21 m||30.0 CH||2.23 m|
|11||132.6 C||132.7 C||132.7 C|
|12||169.6 C||169.6 C||169.5 C|
|13||123.9 CH2||6.27 d (3.0),|
5.62 d (3.0)
|123.8 CH2||6.30 d (3.0)|
5.67 d (3.0)
|123.8 CH2||6.32 d (3.0)|
5.67 d (3.0)
|14||20.0 CH3||0.92 d (7.0)||19.9 CH3||0.94 d (6.6)||20.0 CH3||0.98 d (6.5)|
|15||23.4 CH3||1.18 s||23.3 CH3||1.21 s||23.5 CH3||1.24 s|
|1′||172.5 C||176.3 C||175.9 C|
|2′||42.7 CH2||2.31 d (7.0), 2.26 o||33.9 CH||2.67 m||41.3 CH||2.52 m|
|3′||25.3 CH||2.09 o c||18.0 CH3||1.21 d (6.6)||26.3 CH2||1.76 o, 1.52 o|
|4′||21.3 CH3||0.96 d (6.0)||17.9 CH3||1.20 d (6.6)||16.1 CH3||1.24 d (7.0)|
|5′||21.4 CH3||0.95 d (6.0)||10.7 CH3||0.98 t (7.0)|
|1′′||173.3 C||167.2 C||176.7 C|
|2′′||43.0 CH2||2.26 o, 2.06 o||136.4 C||41.5 CH||2.52 m|
|3′′||25.4 CH||2.09 o||124.7 CH2||5.63 dq (3.6, 1.8),|
6.13 dq (3.6, 1.8)
|26.2 CH2||1.76 o, 1.52 o|
|4′′||21.4 CH3||0.96 d (6.5)||17.1 CH3||1.96 br s||16.2 CH3||1.26 d (7.0)|
|5′′||21.4 CH3||0.95 d (6.5)||10.6 CH3||0.96 t (7.0)|
a Measured at 500 MHz in methanol-d4; b Measured at 600 MHz in methanol-d4; c Overlapped with other signals.
Table 2. In Vitro Cytotoxic Activities of Compounds 1–5.
|1||>40||16.98 ± 2.23||29.39 ± 0.17|
|2||30.70 ± 0.51||7.47 ± 0.21||16.82 ± 0.27|
|5||>40||31.64 ± 0.16||11.63 ± 1.00|
|cis-platin||7.90 ± 0.23||13.03 ± 1.49||15.34 ± 0.35|
Values were mean ± SD; Cis-platin, positive control; Cell lines: A549: lung cancer, Hep G2: hepatocellular cancer, and HeLa: cervical cancer.
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).