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Molecules 2018, 23(5), 1019; https://doi.org/10.3390/molecules23051019

Simultaneous Determination of Decursin, Decursinol Angelate, Nodakenin, and Decursinol of Angelica gigas Nakai in Human Plasma by UHPLC-MS/MS: Application to Pharmacokinetic Study

1
College of Pharmacy, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13488, Korea
2
National Development Institute of Korean Medicine, 288 Udeuraendeu-gil, Anyang-myeon, Jangheung-gun, Jeollanam-do 59338, Korea
3
Department of Oriental Rehabilitation Medicine, Wonkwang University Gwangju Medical Center, 1140-23 Hoejae-ro, Nam-gu, Gwangju 61729, Korea
4
College of Pharmacy, Chonnam National University, 77 Yongbong-ro, Buk-Gu, Gwangju 61186, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Maria Carla Marcotullio
Received: 28 March 2018 / Revised: 20 April 2018 / Accepted: 23 April 2018 / Published: 26 April 2018
(This article belongs to the Special Issue Qualitative and Quantitative Analysis of Bioactive Natural Products)
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Abstract

Coumarins in Cham-dang-gwi, the dried root of Angelica gigas Nakai (AGN), possess pharmacological effects on anemia, pain, infection, and articular rheumatism. The AGN root containes decursin (D), decursinol angelate (DA), nodakenin, and decursinol (DOH), a major metabolite of D and DA. The aim of this study was to develop a simultaneous determination method for these four coumarins in human plasma using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Chromatographic separation was performed on dual columns (Kinetex® C18 column and Capcell core C18 column) with mobile phase consisting of water and acetonitrile at a flow rate of 0.3 mL/min using gradient elution. Multiple reaction monitoring was operated in positive ion mode with precursors to product ion transition values of m/z 328.9→228.8, 328.9→228.9, 409.4→248.8, and 246.8→212.9 to measure D, DA, nodakenin, and DOH, respectively. Linear calibration curves were fitted over concentration range of 0.05–50 ng/mL for these four components, with correlation coefficient greater than 0.995. Inter- and intra-day accuracies were between 90.60% and 108.24%. These precisions were within 11.19% for all components. The established method was then applied to a pharmacokinetic study for the four coumarins after usual dosing in Korean subjects. View Full-Text
Keywords: UHPLC-MS/MS; decursin; decursinol angelate; nodakenin; decursinol UHPLC-MS/MS; decursin; decursinol angelate; nodakenin; decursinol
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Kim, S.-J.; Ko, S.-M.; Choi, E.-J.; Ham, S.-H.; Kwon, Y.-D.; Lee, Y.-B.; Cho, H.-Y. Simultaneous Determination of Decursin, Decursinol Angelate, Nodakenin, and Decursinol of Angelica gigas Nakai in Human Plasma by UHPLC-MS/MS: Application to Pharmacokinetic Study. Molecules 2018, 23, 1019.

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