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Open AccessFeature PaperArticle

Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection

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Department of Chemistry, Boston University, Boston, MA 02215, USA
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Departments of Biomedical Engineering and Medicine, Boston University, Boston, MA 02215, USA
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Authors to whom correspondence should be addressed.
Academic Editor: Ashok Kakkar
Molecules 2018, 23(4), 936; https://doi.org/10.3390/molecules23040936
Received: 21 March 2018 / Revised: 10 April 2018 / Accepted: 11 April 2018 / Published: 18 April 2018
Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available. View Full-Text
Keywords: dendrimer; hydrogel; screening; crosslinking; fluorescence dendrimer; hydrogel; screening; crosslinking; fluorescence
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MDPI and ACS Style

Christadore, L.; Grinstaff, M.W.; Schaus, S.E. Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection. Molecules 2018, 23, 936.

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